Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0948 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0948 |
| Research group | RG Outeiro |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | TSG101 |
| Antibody Registry ID(s) | AB_2208084 |
| Name | Anti-TSG101 antibody |
| Alternative name | VPS23 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | mouse, rat, human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide corresponding to Human TSG101 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab30871 |
| Lot no. | |
| Description | WB Use a concentration of 1 µg/ml. Detects a band of approximately 48,49 kDa (predicted molecular weight: 44 kDa).Can be blocked with TSG101 peptide (ab30870). The doublet seen in Western blot has been described in PMID:11427703 and may be due to internal initiation at Met10. ICC/IF Use a concentration of 5 µg/ml. IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| Localization | Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2022-02-26 00:00:00 |
| Preparation date | 2022-02-26 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=TSG101 |
| Wikipedia | http://en.wikipedia.org/wiki/TSG101 |
| Uniprot | http://www.uniprot.org/uniprot/?query=TSG101 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=TSG101 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2208084 |
Applications
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Linked publications
Ectosomes and exosomes are distinct proteomic entities that modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Riedel D, Parfentev I, Gerhardt E, Urlaub H, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.06.24.449731
