View primary antibody

Basic data

PIDprimary-0841
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0841
Research groupRG Rehling
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolOXPHOS
Antibody Registry ID(s)AB_2629281
NameTotal OXPHOS Rodent WB Antibody Cocktail
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Cow, Human, CynomolgusMonkey
Clone
Isotype
Clonalitymonoclonal
Demaskingunknown
Antigen
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab110413
Lot no.
DescriptionThis OXPHOS cocktail contains 5 mouse mAbs, one each against CI subunit NDUFB8 (ab110242), CII-30kDa (ab14714), CIII-Core protein 2 (ab14745), CIV subunit I (ab14705) and CV alpha subunit (ab14748) as an optimized premixed cocktail. WB Use at an assay dependent concentration. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6.0 µg/mL for Western blotting. We recommend using PBS with 1% milk as the antibody diluent. Store the antibody cocktail at 4°C and the control sample at -80°C. We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.
Localization
Storage instructionStore at -80°C. Please refer to protocols.
Receipt date2021-11-07 00:00:00
Preparation date2021-11-07 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Loss of Mitochondrial Ca2+ Uniporter Limits Inotropic Reserve and Provides Trigger and Substrate for Arrhythmias in Barth Syndrome Cardiomyopathy
Bertero E, Nickel A, Kohlhaas M, Hohl M, Sequeira V, Brune C, Schwemmlein J, Abeßer M, Schuh K, Kutschka I, Carlein C, Münker K, Atighetchi S, Müller A, Kazakov A, Kappl R, von der Malsburg K, van der Laan M, Schiuma A, Böhm M, Laufs U, Hoth M, Rehling P, Kuhn M, Dudek J, von der Malsburg A, Prates Roma L, Maack C
Circulation 2021 : 10.1161/circulationaha.121.053755