Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0807 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0807 |
| Research group | RG Cramer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | POLR2A |
| Antibody Registry ID(s) | AB_630203 |
| Name | Anti-Pol II Antibody |
| Alternative name | DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | mouse, rat, human |
| Clone | |
| Isotype | IgG2b |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | raised against amino acids 1-224 of Pol II of human origin |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-55492 |
| Lot no. | |
| Description | Pol II (F-12) is recommended for detection of Pol II of mouse, rat and humanorigin by Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 mlof cell lysate)], immunofluorescence (starting dilution 1:50, dilution range1:50-1:500), immunohistochemistry (including paraffin-embedded sections)(starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA(starting dilution 1:30, dilution range 1:30-1:3000). |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required. |
| Receipt date | 2021-10-23 00:00:00 |
| Preparation date | 2021-10-23 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=POLR2A |
| Wikipedia | http://en.wikipedia.org/wiki/POLR2A |
| Uniprot | http://www.uniprot.org/uniprot/?query=POLR2A |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=POLR2A |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_630203 |
Applications
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Linked publications
Structural basis of human transcription–DNA repair coupling
Kokic G, Wagner FR, Chernev A, Urlaub H, Cramer P
Nature 2021 : 10.1038/s41586-021-03906-4
