View primary antibody

Basic data

PIDprimary-0795
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0795
Research groupRG Zimmermann
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTNNT2
Antibody Registry ID(s)AB_956386
NameAnti-Cardiac Troponin T antibody
Alternative nameCMPD2
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withHuman
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
Antigen Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human cardiac Troponin T.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab45932
Lot no.
DescriptionIHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) as Capture. WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
Localization
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-10-13 00:00:00
Preparation date2021-10-13 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression
Schoger E, Zimmermann W, Cyganek L, Zelarayán LC
Stem Cell Res 2021 : 10.1016/j.scr.2021.102473

Establishment of a second generation homozygous CRISPRa human induced pluripotent stem cell (hiPSC) line for enhanced levels of endogenous gene activation
Schoger E, Zimmermann W, Cyganek L, Zelarayán LC
Stem Cell Res 2021 : 10.1016/j.scr.2021.102518

An Alternative Mechanism of Subcellular Iron Uptake Deficiency in Cardiomyocytes
Dai Y, Ignatyeva N, Xu H, Wali R, Toischer K, Brandenburg S, Lenz C, Pronto J, Fakuade FE, Sossalla S, Zeisberg EM, Janshoff A, Kutschka I, Voigt N, Urlaub H, Rasmussen TB, Mogensen J, Lehnart SE, Hasenfuss G, Ebert A
Circ Res 2023 : 10.1161/circresaha.122.321157

Generation of Pelizaeus-Merzbacher disease (PMD) mutant (PLP1-C33Y) in induced pluripotent stem cell (iPSC) by CRISPR/Cas9 genome editing
Schreiber M, Zafeiriou M
Stem Cell Res 2023 : 10.1016/j.scr.2023.103276

Generation of a fluorescent oligodendrocyte reporter line in human induced pluripotent stem cells
Schreiber M, Zafeiriou M
Stem Cell Res 2023 : 10.1016/j.scr.2023.103295