Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0792 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0792 |
| Research group | RG Zimmermann |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | ACTA2 |
| Antibody Registry ID(s) | AB_476701 |
| Name | Monoclonal Anti-Actin antibody |
| Alternative name | ACTSA |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | human, frog, sheep, chicken, goat, bovine, rat, guineaPig, mouse, canine, rabbit, snake |
| Clone | 1A4 |
| Isotype | IgG2a |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | N-terminal synthetic decapeptide of α-smooth muscle actin. |
| Crafted By | company |
| Company / Manufacturer | Sigma-Aldrich |
| Catalog no. | A2547 |
| Lot no. | |
| Description | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells immunohistochemistry (frozen sections): suitable using smooth muscle cells indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix western blot: suitable using smooth muscle cells |
| Localization | |
| Storage instruction | For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| Receipt date | 2021-10-11 00:00:00 |
| Preparation date | 2021-10-11 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
Applications
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Linked publications
Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression
Schoger E, Zimmermann W, Cyganek L, Zelarayán LC
Stem Cell Res 2021 : 10.1016/j.scr.2021.102473
Establishment of a second generation homozygous CRISPRa human induced pluripotent stem cell (hiPSC) line for enhanced levels of endogenous gene activation
Schoger E, Zimmermann W, Cyganek L, Zelarayán LC
Stem Cell Res 2021 : 10.1016/j.scr.2021.102518
Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V
Busley AV, Kleinsorge M, Cyganek L
Stem Cell Res 2023 : 10.1016/j.scr.2023.103105
Generation of a genetically-modified induced pluripotent stem cell line harboring a Noonan syndrome-associated gene variant MRAS p.G23V
Busley AV, Cyganek L
Stem Cell Res 2023 : 10.1016/j.scr.2023.103108
Generation of a pluripotent stem cell line (UMGi270-A) and a corresponding CRISPR/Cas9 modified isogenic control (UMGi270-A-1) from a patient with sudden onset dilated cardiomyopathy harboring a FLNC p.R2187P mutation
Maurer W, Rebs S, Köhne S, Eberl H, Wollnik B, Zibat A, Streckfuss-Bömeke K
Stem Cell Res 2024 : 10.1016/j.scr.2024.103409
