Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0790 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0790 |
| Research group | RG Zimmermann |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | POU5F1 |
| Antibody Registry ID(s) | AB_445175 |
| Name | Anti-Oct4 antibody |
| Alternative name | MGC22487 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | Mouse, Human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Syn. peptide corresponding to Human Oct4 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab19857 |
| Lot no. | |
| Description | IP Use at an assay dependent concentration. ICC/IF Use a concentration of 1 - 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 39 kDa). |
| Localization | Nucleus. Expressed in a diffuse and slightly punctuate pattern. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-10-09 00:00:00 |
| Preparation date | 2021-10-09 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=POU5F1 |
| Wikipedia | http://en.wikipedia.org/wiki/POU5F1 |
| Uniprot | http://www.uniprot.org/uniprot/?query=POU5F1 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=POU5F1 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_445175 |
Applications
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Linked publications
Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression
Schoger E, Zimmermann W, Cyganek L, Zelarayán LC
Stem Cell Res 2021 : 10.1016/j.scr.2021.102473
Establishment of a second generation homozygous CRISPRa human induced pluripotent stem cell (hiPSC) line for enhanced levels of endogenous gene activation
Schoger E, Zimmermann W, Cyganek L, Zelarayán LC
Stem Cell Res 2021 : 10.1016/j.scr.2021.102518
