View primary antibody

Basic data

PID primary-0777
EPIC PID
Research group RG Cramer
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol POLR2A
Antibody Registry ID(s) AB_2801296
Name Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody
Alternative name DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
Lab ID
Tag / Fluorophore
Raised in
Reacts with mouse
Clone 3E8
Isotype IgG2aκ
Clonality monoclonal
Demasking unknown
Antigen linear peptide corresponding to human RNA POLR2A phosphorylated at Ser5 of the C-terminal domain (CTD).
Crafted By company
Company / Manufacturer Merck Millipore
Catalog no. 04-1572-I
Lot no.
Description Key Applications Chromatin Immunoprecipitation (ChIP) Western Blotting ELISA
Localization
Storage instruction Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Receipt date 2021-10-02 00:00:00
Preparation date 2021-10-02 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

External links

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Linked publications


Structure of the human Mediator–RNA polymerase II pre-initiation complex
Rengachari S et al.
Nature 2021: 10.1038/s41586-021-03555-7