Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0777 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0777 |
| Research group | RG Cramer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | POLR2A |
| Antibody Registry ID(s) | AB_2801296 |
| Name | Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody |
| Alternative name | DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rat |
| Reacts with | mouse |
| Clone | 3E8 |
| Isotype | IgG2aκ |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | linear peptide corresponding to human RNA POLR2A phosphorylated at Ser5 of the C-terminal domain (CTD). |
| Crafted By | company |
| Company / Manufacturer | Merck Millipore |
| Catalog no. | 04-1572-I |
| Lot no. | |
| Description | Key Applications Chromatin Immunoprecipitation (ChIP) Western Blotting ELISA |
| Localization | |
| Storage instruction | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Receipt date | 2021-10-02 00:00:00 |
| Preparation date | 2021-10-02 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
Applications
No application comments yet.
Linked publications
Structure of the human Mediator–RNA polymerase II pre-initiation complex
Rengachari S, Schilbach S, Aibara S, Dienemann C, Cramer P
Nature 2021 : 10.1038/s41586-021-03555-7
