View primary antibody

Basic data

PIDprimary-0660
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0660
Research groupRG Outeiro
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolNEFH
Antibody Registry ID(s)AB_477257
NameAnti-Neurofilament 200 (Phos. and Non-Phos.) antibody
Alternative nameNFH
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withwideRange
CloneN52
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenC-terminal segment of enzymatically dephosphorylated pig neurofilament 200.
Crafted Bycompany
Company / ManufacturerSigma-Aldrich
Catalog no.N0142
Lot no.
Descriptionapplication(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunohistochemistry (frozen sections): 1:400 using rat cerebellum microarray: suitable western blot: 1:1,000 using rat brain extract
Localization
Storage instructionFor continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Receipt date2021-08-12 00:00:00
Preparation date2021-08-12 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Super-resolution microscopy informs on the molecular architecture of alpha-synuclein inclusions in model systems and in the human brain
Weish P, Lazaro DF, Palmares L, Santos PI, Stadelmann C, Hoeglinger GU, Rizzoli SO, Outeiro TF
bioRxiv 2021 : 10.1101/2021.04.25.441304

A new form of axonal pathology in a spinal model of neuromyelitis optica
Herwerth M, Kenet S, Schifferer M, Winkler A, Weber M, Snaidero N, Wang M, Lohrberg M, Bennett JL, Stadelmann C, Hemmer B, Misgeld T
Brain 2022 : 10.1093/brain/awac079