View primary antibody

Basic data

PIDprimary-0602
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0602
Research groupRG Fernandez-Busnadiego
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolHTT
Antibody Registry ID(s)AB_532270
NameAnti-Polyglutamines antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withHuman
Clone3B5H10
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
Antigen
Crafted Bycompany
Company / ManufacturerSigma-Aldrich
Catalog no.P1874
Lot no.
Descriptionimmunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: 1-2 μg/mL using extract of HEK-293T cells transfected with an N-terminal 171 amino acid fragment of human Huntingtin with a 68 glutamine stretch
Localization
Storage instructionstorage temp. −20°C
Receipt date2021-07-20 00:00:00
Preparation date2021-07-20 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


PolyQ expansion does not alter the Huntingtin-HAP40 complex
Huang B, Guo Q, Niedermeier ML, Cheng J, Engler T, Maurer M, Pautsch A, Baumeister W, Stengel F, Kochanek S, Fernández-Busnadiego R
bioRxiv 2021 : 10.1101/2021.02.02.429316

Pathological polyQ expansion does not alter the conformation of the Huntingtin-HAP40 complex
Huang B, Guo Q, Niedermeier ML, Cheng J, Engler T, Maurer M, Pautsch A, Baumeister W, Stengel F, Kochanek S, Fernández-Busnadiego R
Structure 2021 : 10.1016/j.str.2021.04.003