Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0556 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0556 |
| Research group | RG Fischer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | EOMES |
| Antibody Registry ID(s) | AB_778267 |
| Name | Anti-TBR2 / Eomes antibody |
| Alternative name | T-box brain2 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | mouse, human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Corresponding to Mouse Eomes aa 650 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab23345 |
| Lot no. | |
| Description | IHC-Fr 1/100 - 1/500. Abcam recommends the following antigen retrieval method: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody. WB Use a concentration of 0.4 - 2.5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 72 kDa). Abcam recommends using milk as the blocking agent. In our hands, when tested in western blot, this product typically gives a weaker signal in mouse tissue lysates compared to human cell lines. However, this product gives clean, specific staining in IHC-Fr on mouse E14.5 cortex. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented. |
| Localization | Nucleus. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-05-23 00:00:00 |
| Preparation date | 2021-05-23 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=EOMES |
| Wikipedia | http://en.wikipedia.org/wiki/EOMES |
| Uniprot | http://www.uniprot.org/uniprot/?query=EOMES |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=EOMES |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_778267 |
Applications
No application comments yet.
Linked publications
Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex
Ulmke PA, Sakib MS, Ditte P, Sokpor G, Kerimoglu C, Pham L, Xie Y, Mao X, Rosenbusch J, Teichmann U, Nguyen HP, Fischer A, Eichele G, Staiger JF, Tuoc T
Stem Cell Reports 2021 : 10.1016/j.stemcr.2021.03.008
