View primary antibody

Basic data

PIDprimary-0556
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0556
Research groupRG Fischer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolEOMES
Antibody Registry ID(s)AB_778267
NameAnti-TBR2 / Eomes antibody
Alternative nameT-box brain2
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withMouse, Human
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenCorresponding to Mouse Eomes aa 650 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab23345
Lot no.
DescriptionIHC-Fr 1/100 - 1/500. Abcam recommends the following antigen retrieval method: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody. WB Use a concentration of 0.4 - 2.5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 72 kDa). Abcam recommends using milk as the blocking agent. In our hands, when tested in western blot, this product typically gives a weaker signal in mouse tissue lysates compared to human cell lines. However, this product gives clean, specific staining in IHC-Fr on mouse E14.5 cortex. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented.
LocalizationNucleus.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-05-23 00:00:00
Preparation date2021-05-23 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex
Ulmke PA, Sakib MS, Ditte P, Sokpor G, Kerimoglu C, Pham L, Xie Y, Mao X, Rosenbusch J, Teichmann U, Nguyen HP, Fischer A, Eichele G, Staiger JF, Tuoc T
Stem Cell Reports 2021 : 10.1016/j.stemcr.2021.03.008