View primary antibody

Basic data

PIDprimary-0545
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0545
Research groupRG Jakobs
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolATP5F1B
Antibody Registry ID(s)AB_304883
NameAnti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker
Alternative nameATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Human
Clone4.3E8.D10
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenOther Immunogen Type corresponding to ATPB. Intact rat mitochondria.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab5432
Lot no.GR3177231-2
DescriptionWB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). ICC/IF 1/100 - 1/1000. IP Use a concentration of 2 - 5 µg/ml. By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria.
LocalizationMitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-05-20 00:00:00
Preparation date2021-05-20 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

External links

Applications


No application comments yet.

Linked publications


Colocalization for super-resolution microscopy via optimal transport
Tameling C, Stoldt S, Stephan T, Naas J, Jakobs S, Munk A
Nat Comput Sci 2021 : 10.1038/s43588-021-00050-x

DNA-PAINT MINFLUX nanoscopy
Ostersehlt LM, Jans DC, Wittek A, Keller-Findeisen J, Inamdar K, Sahl SJ, Hell SW, Jakobs S
Nat Methods 2022 : 10.1038/s41592-022-01577-1