View primary antibody

Basic data

PIDprimary-0537
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0537
Research groupRG Outeiro
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolGAPDH
Antibody Registry ID(s)AB_2107296
NameAnti-GAPDH Antibody (H-12)
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Human
Clone
IsotypeIgG2a κ
Clonalitymonoclonal
Demaskingunknown
Antigenraised against a peptide mapping within an internal region of GAPDH of human origin
Crafted Bycompany
Company / ManufacturerSanta Cruz Biotechnology
Catalog no.sc-166574
Lot no.
DescriptionAPPLICATIONS: GAPDH (H-12) is recommended for detection of GAPDH of mouse, rat andhuman origin by Western Blotting (starting dilution 1:100, dilution range1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500), immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phaseELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Localization
Storage instructionStore at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.
Receipt date2021-05-18 00:00:00
Preparation date2021-05-18 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Cofilin pathology is a new player on α-synuclein-induced spine impairment in models of hippocampal synucleinopathy
Oliveira da Silva MI, Santejo M, Babcock I, Magalhães A, Minamide L, Castillo E, Gerhardt E, Fahlbusch C, Swanson R, Outeiro T, Bamburg J, Liz M
bioRxiv 2021 : 10.1101/2021.02.02.425931