View primary antibody

Basic data

PID primary-0537
EPIC PID
Research group RG Outeiro
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol GAPDH
Antibody Registry ID(s) AB_2107296
Name Anti-GAPDH Antibody (H-12)
Alternative name
Lab ID
Tag / Fluorophore
Raised in
Reacts with mouse, rat, human
Clone
Isotype IgG2a κ
Clonality monoclonal
Demasking unknown
Antigen raised against a peptide mapping within an internal region of GAPDH of human origin
Crafted By company
Company / Manufacturer Santa Cruz Biotechnology
Catalog no. sc-166574
Lot no.
Description APPLICATIONS: GAPDH (H-12) is recommended for detection of GAPDH of mouse, rat andhuman origin by Western Blotting (starting dilution 1:100, dilution range1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500), immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phaseELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Localization
Storage instruction Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.
Receipt date 2021-05-18 00:00:00
Preparation date 2021-05-18 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

External links

Antibodypedia -
HGNC https://www.genenames.org/tools/search/#!/?query=GAPDH
Wikipedia https://en.wikipedia.org/wiki/GAPDH
Uniprot https://www.uniprot.org/uniprot/?query=GAPDH
GeneCards https://www.genecards.org/cgi-bin/carddisp.pl?gene=GAPDH
Antibody Registry https://antibodyregistry.org/search?q=AB_2107296

Applications

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Linked publications

Cofilin pathology is a new player on α-synuclein-induced spine impairment in models of hippocampal synucleinopathy
Oliveira da Silva M et al.
bioRxiv 2021: 10.1101/2021.02.02.425931