View primary antibody

Basic data

PIDprimary-0506
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0506
Research groupRG Fischer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolPAX6
Antibody Registry ID(s)AB_528427
NamePax6 antibody
Alternative nameAniridia 1
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withamphibian, avian, fish, human, lizard, mouse, Opossum, Planaria, rat, turtle, zebrafish
Clone
IsotypeMIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenRecombinant partial protein (N-terminal region, aa 1-223)
Crafted Bycompany
Company / ManufacturerDSHB
Catalog no.pax6
Lot no.
DescriptionAlthough the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Localization
Storage instructionFor immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Receipt date2021-05-12 00:00:00
Preparation date2021-05-12 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Post-transcriptional regulation by the exosome complex is required for cell survival and forebrain development via repression of P53 signaling
Ulmke PA, Xie Y, Sokpor G, Pham L, Shomroni O, Berulava T, Rosenbusch J, Basu U, Fischer A, Nguyen HP, Staiger JF, Tuoc T
Development 2021 : 10.1242/dev.188276