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University Medical Center Göttingen
Imprint
| PID | primary-0496 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0496 |
| Research group | RG Cramer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
| Antigen symbol | SNRNP70 |
| Antibody Registry ID(s) | AB_2801569 |
| Name | Anti-U1 snRNP 70 Antibody (C-3) |
| Alternative name | U1-70K |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | mouse, human, rat |
| Clone | C-3 |
| Isotype | IgG1 |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | specific for an epitope mapping between amino acids 183-280 of U1 snRNP 70 of human origin |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-390899 |
| Lot no. | |
| Description | U1 snRNP 70 (C-3) is recommended for detection of U1 snRNP 70 of mouse, rat and human origin by Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE** |
| Receipt date | 2021-05-07 00:00:00 |
| Preparation date | 2021-05-07 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=SNRNP70 |
| Wikipedia | http://en.wikipedia.org/wiki/SNRNP70 |
| Uniprot | http://www.uniprot.org/uniprot/?query=SNRNP70 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=SNRNP70 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2801569 |
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Transcription activation depends on the length of the RNA polymerase II C‐terminal domain
Sawicka A, Villamil G, Lidschreiber M, Darzacq X, Dugast‐Darzacq C, Schwalb B, Cramer P
EMBO J 2021 : 10.15252/embj.2020107015
Structure of a transcribing RNA polymerase II–U1 snRNP complex
Zhang S, Aibara S, Vos SM, Agafonov DE, Lührmann R, Cramer P
Science 2021 : 10.1126/science.abf1870