View primary antibody

Basic data

PIDprimary-0496
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0496
Research groupRG Cramer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolSNRNP70
Antibody Registry ID(s)AB_2801569
NameAnti-U1 snRNP 70 Antibody (C-3)
Alternative nameU1-70K
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Human, Rat
CloneC-3
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
Antigenspecific for an epitope mapping between amino acids 183-280 of U1 snRNP 70 of human origin
Crafted Bycompany
Company / ManufacturerSanta Cruz Biotechnology
Catalog no.sc-390899
Lot no.
DescriptionU1 snRNP 70 (C-3) is recommended for detection of U1 snRNP 70 of mouse, rat and human origin by Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Localization
Storage instructionStore at 4° C, **DO NOT FREEZE**
Receipt date2021-05-07 00:00:00
Preparation date2021-05-07 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Transcription activation depends on the length of the RNA polymerase II C‐terminal domain
Sawicka A, Villamil G, Lidschreiber M, Darzacq X, Dugast‐Darzacq C, Schwalb B, Cramer P
EMBO J 2021 : 10.15252/embj.2020107015

Structure of a transcribing RNA polymerase II–U1 snRNP complex
Zhang S, Aibara S, Vos SM, Agafonov DE, Lührmann R, Cramer P
Science 2021 : 10.1126/science.abf1870