View primary antibody

Basic data

PIDprimary-0483
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0483
Research groupRG Cramer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolNELFCD
Antibody Registry ID(s)AB_2847956
NameAnti-NELF-D Antibody (C-10)
Alternative nametrihydrophobin 1
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Human
CloneC-10
IsotypeIgG2a
Clonalitymonoclonal
Demaskingunknown
Antigenraised against amino acids 1-300 mapping at the N-terminus of NELF-D of human origin
Crafted Byunknown
Company / Manufacturer
Catalog no.
Lot no.
DescriptionNELF-D (C-10) is recommended for detection of NEFL-D of mouse, rat and human origin by Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Localization
Storage instructionStore at 4° C, **DO NOT FREEZE**
Receipt date2021-05-02 00:00:00
Preparation date2021-05-02 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Stress-induced nuclear condensation of NELF drives transcriptional downregulation
Rawat P, Boehning M, Hummel B, Aprile-Garcia F, Pandit AS, Eisenhardt N, Khavaran A, Niskanen E, Vos SM, Palvimo JJ, Pichler A, Cramer P, Sawarkar R
Mol Cell 2021 : 10.1016/j.molcel.2021.01.016