View primary antibody

Basic data

PIDprimary-0467
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0467
Research groupRG Hasenfuß
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolSLC8A1
Antibody Registry ID(s)AB_2721228
NameRabbit anti Na+ -Ca2+-exchanger
Alternative name
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withcanine, mouse, rat, rabbit, frog, toad, guineaPig, cow, human
Clone
Isotype
Clonalitypolyclonal
Demaskingunknown
AntigenProduced against canine cardiac sarcolemmal Na+ -Ca2+ exchanger (NCX1; full length isolated protein).
Crafted Bycompany
Company / ManufacturerSwant
Catalog no.π 11-13
Lot no.28
DescriptionImmunohistochemistry; 1:5’000 - 1: 10’000 with the avidin-biotin method Immunoblots: 1:100 – 1: 1’000 (use milk for blocking). Primary bands at 120 kDa (full length protein), 160 kDA (some non-reduced protein even in the presence of DDT) and 70 kDa (active proteolytic fragment). We recommend that the optimal dilutions be determined by titration experiments
Localization
Storage instructionFor long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
Receipt date2021-04-29 00:00:00
Preparation date2021-04-29 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes
Peper J, Kownatzki-Danger D, Weninger G, Seibertz F, Pronto JR, Sutanto H, Pacheu Grau D, Hindmarsh R, Brandenburg S, Kohl T, Hasenfuβ G, Gotthardt M, Rog-Zielinska EA, Wollnik B, Rehling P, Urlaub H, Wegener JW, Heijman J, Voigt N, Cyganek L, Lenz C, Lehnart SE
Circ Res 2021 : 10.1161/circresaha.119.316547