View primary antibody

Basic data

PIDprimary-0405
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0405
Research groupRG Wollnik
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbol NCAM1
Antibody Registry ID(s)AB_528389
NameAnti-NCAM Antibody
Alternative nameCD56
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withavian, chicken, frog, Xenopus
Clone
IsotypeMIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenNCAM (cytoplasmic domain), 180 kDa isoform
Crafted Bycompany
Company / ManufacturerDSHB
Catalog no.4d
Lot no.
DescriptionAlthough the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Localization
Storage instructionAlthough many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Receipt date2021-04-23 00:00:00
Preparation date2021-04-23 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome
Ufartes R, Berger H, Till K, Salinas G, Sturm M, Altmüller J, Nürnberg P, Thiele H, Funke R, Apeshiotis N, Langen H, Wollnik B, Borchers A, Pauli S
Hum Genet 2020 : 10.1007/s00439-020-02175-x