View primary antibody

Basic data

PIDprimary-0404
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0404
Research groupRG Wollnik
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolCOL2A1
Antibody Registry ID(s)AB_528165
NameAnti- collagen type II Antibody
Alternative namecollagen, type II, alpha 1
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withchicken, avian, bovine, fish, goat, human, mouse, Ovine, Quail, rabbit, rat, shark, Xenopus, zebrafish
Clone
IsotypeMIgG1
Clonalitymonoclonal
Demaskingunknown
Antigen Collagen type II, Full length protein
Crafted Bycompany
Company / ManufacturerDSHB
Catalog no.II-II6B3
Lot no.
DescriptionAlthough the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Localization
Storage instructionAlthough many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Receipt date2021-04-23 00:00:00
Preparation date2021-04-23 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome
Ufartes R, Berger H, Till K, Salinas G, Sturm M, Altmüller J, Nürnberg P, Thiele H, Funke R, Apeshiotis N, Langen H, Wollnik B, Borchers A, Pauli S
Hum Genet 2020 : 10.1007/s00439-020-02175-x