View primary antibody

Basic data

PIDprimary-0385
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0385
Research groupRG Griesinger
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolACTB
Antibody Registry ID(s)AB_476744
NameMonoclonal Anti-β-Actin antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withpig, bovine, rat, canine, feline, human, rabbit, carp, mouse, guineaPig, chicken, sheep
CloneAC-15
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
Antigenslightly modified β-cytoplasmic actin N-terminal peptide
Crafted Bycompany
Company / ManufacturerSigma-Aldrich
Catalog no.A5441
Lot no.
Description immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable indirect ELISA: suitable indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts
Localization
Storage instructionstorage temp. −20°C
Receipt date2021-04-22 00:00:00
Preparation date2021-04-22 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Effects of pharmacological modulators of α-synuclein and tau aggregation and internalization
Dominguez-Meijide A, Vasili E, König A, Cima-Omori M, Ibáñez de Opakua A, Leonov A, Ryazanov S, Zweckstetter M, Griesinger C, Outeiro TF
Sci Rep 2020 : 10.1038/s41598-020-69744-y

Human RAD50 deficiency: Confirmation of a distinctive phenotype
Ragamin A, Yigit G, Bousset K, Beleggia F, Verheijen FW, Wit MY, Strom TM, Dörk T, Wollnik B, Mancini GMS
Am J Med Genet A 2020 : 10.1002/ajmg.a.61570

Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825

Monitoring Alpha-Synuclein – Tau Interactions In Vitro and In Vivo Using Bimolecular Fluorescence Complementation
Torres-Garcia L, Domingues JM, Brandi E, Haikal C, Brás IC, Gerhardt E, Li W, Svanbergsson A, Outeiro TF, Gouras GK, Li J
Researchsquare 2021 : 10.21203/rs.3.rs-960446/v1

Ectosomes and exosomes are distinct proteomic entities that modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Riedel D, Parfentev I, Gerhardt E, Urlaub H, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.06.24.449731

Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation
Torres-Garcia L, P. Domingues JM, Brandi E, Haikal C, Mudannayake JM, Brás IC, Gerhardt E, Li W, Svanbergsson A, Outeiro TF, Gouras GK, Li J
Sci Rep 2022 : 10.1038/s41598-022-06846-9