Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0378 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0378 |
| Research group | RG Fischer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | H3C |
| Antibody Registry ID(s) | AB_2118291 |
| Name | Anti-Histone H3 (acetyl K27) antibody - ChIP Grade |
| Alternative name | H3 histone family member E pseudogene antibody |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | Mouse, Cow, Human, RecombinantFragment |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide corresponding to Human Histone H3 aa 1-100 (acetyl K27) conjugated to keyhole limpet haemocyanin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab4729 |
| Lot no. | |
| Description | ICC/IF Use a concentration of 0.5 µg/ml. Can be used with paraformaldehyde- or methanol- fixed cells. WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ChIP Use 2 µg for 25 µg of chromatin. We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control. PepArr Use a concentration of 0.2 - 0.02 µg/ml. |
| Localization | Nucleus. Chromosome. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-04-21 00:00:00 |
| Preparation date | 2021-04-21 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=H3C |
| Wikipedia | http://en.wikipedia.org/wiki/H3C |
| Uniprot | http://www.uniprot.org/uniprot/?query=H3C |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=H3C |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2118291 |
Applications
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Linked publications
SETD1B controls cognitive function via cell type specific regulation of neuronal identity genes
Michurina A, Sakib S, Kerimoglu C, Krüger DM, Kaurani L, Islam R, Centeno TP, Cha J, Xu X, Zeisberg EM, Kranz A, Stewart FA, Fischer A
bioRxiv 2020 : 10.1101/2020.08.07.240853
Postnatal expression of the lysine methyltransferase SETD1B is essential for learning and the regulation of neuron-enriched genes
Michurina A, Sakib MS, Kerimoglu C, Krüger DM, Kaurani L, Islam MR, Joshi PD, Schröder S, Centeno TP, Zhou J, Pradhan R, Cha J, Xu X, Eichele G, Zeisberg EM, Kranz A, Stewart AF, Fischer A
EMBO J 2021 : 10.15252/embj.2020106459
