View primary antibody

Basic data

PIDprimary-0378
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0378
Research groupRG Fischer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolH3C
Antibody Registry ID(s)AB_2118291
NameAnti-Histone H3 (acetyl K27) antibody - ChIP Grade
Alternative nameH3 histone family member E pseudogene antibody
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withmouse, cow, human, RecombinantFragment
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenSynthetic peptide corresponding to Human Histone H3 aa 1-100 (acetyl K27) conjugated to keyhole limpet haemocyanin.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab4729
Lot no.
DescriptionICC/IF Use a concentration of 0.5 µg/ml. Can be used with paraformaldehyde- or methanol- fixed cells. WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ChIP Use 2 µg for 25 µg of chromatin. We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control. PepArr Use a concentration of 0.2 - 0.02 µg/ml.
LocalizationNucleus. Chromosome.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-04-21 00:00:00
Preparation date2021-04-21 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


SETD1B controls cognitive function via cell type specific regulation of neuronal identity genes
Michurina A, Sakib S, Kerimoglu C, Krüger DM, Kaurani L, Islam R, Centeno TP, Cha J, Xu X, Zeisberg EM, Kranz A, Stewart FA, Fischer A
bioRxiv 2020 : 10.1101/2020.08.07.240853

Postnatal expression of the lysine methyltransferase SETD1B is essential for learning and the regulation of neuron-enriched genes
Michurina A, Sakib MS, Kerimoglu C, Krüger DM, Kaurani L, Islam MR, Joshi PD, Schröder S, Centeno TP, Zhou J, Pradhan R, Cha J, Xu X, Eichele G, Zeisberg EM, Kranz A, Stewart AF, Fischer A
EMBO J 2021 : 10.15252/embj.2020106459