| Antigen symbol |
H3C |
| Antibody Registry ID(s) |
AB_2118291 |
| Name |
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade |
| Alternative name |
H3 histone family member E pseudogene antibody |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
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| Reacts with |
mouse, cow, human, RecombinantFragment |
| Clone |
|
| Isotype |
IgG |
| Clonality |
polyclonal |
| Demasking |
unknown |
| Antigen |
Synthetic peptide corresponding to Human Histone H3 aa 1-100 (acetyl K27) conjugated to keyhole limpet haemocyanin. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab4729 |
| Lot no. |
|
| Description |
ICC/IF Use a concentration of 0.5 µg/ml. Can be used with paraformaldehyde- or methanol- fixed cells.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP Use 2 µg for 25 µg of chromatin. We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control.
PepArr Use a concentration of 0.2 - 0.02 µg/ml. |
| Localization |
Nucleus. Chromosome. |
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date |
2021-04-21 00:00:00 |
| Preparation date |
2021-04-21 00:00:00 |
| Created by |
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| Last modified |
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