| Antigen symbol |
H3C1 |
| Antibody Registry ID(s) |
AB_306847 |
| Name |
Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade |
| Alternative name |
H3 histone family member E pseudogene antibody |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
cow, human |
| Clone |
|
| Isotype |
IgG |
| Clonality |
polyclonal |
| Demasking |
unknown |
| Antigen |
Synthetic peptide within Human Histone H3 aa 1-100 (mono methyl K4) conjugated to keyhole limpet haemocyanin. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab8895 |
| Lot no. |
|
| Description |
ICC/IF Use a concentration of 1 µg/ml. Works better if cells are fixed with methanol.
ChIP Use 2 µg for 25 µg of chromatin. We recommend Myo-D ChIP primer pair ab269261 as positive control.
WB 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Localization |
Nucleus. Chromosome. |
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date |
2021-04-21 00:00:00 |
| Preparation date |
2021-04-21 00:00:00 |
| Created by |
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| Last modified |
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