Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0377 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0377 |
| Research group | RG Fischer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | H3C1 |
| Antibody Registry ID(s) | AB_306847 |
| Name | Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade |
| Alternative name | H3 histone family member E pseudogene antibody |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | cow, human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide within Human Histone H3 aa 1-100 (mono methyl K4) conjugated to keyhole limpet haemocyanin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab8895 |
| Lot no. | |
| Description | ICC/IF Use a concentration of 1 µg/ml. Works better if cells are fixed with methanol. ChIP Use 2 µg for 25 µg of chromatin. We recommend Myo-D ChIP primer pair ab269261 as positive control. WB 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Localization | Nucleus. Chromosome. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-04-21 00:00:00 |
| Preparation date | 2021-04-21 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=H3C1 |
| Wikipedia | http://en.wikipedia.org/wiki/H3C1 |
| Uniprot | http://www.uniprot.org/uniprot/?query=H3C1 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=H3C1 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_306847 |
Applications
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Linked publications
SETD1B controls cognitive function via cell type specific regulation of neuronal identity genes
Michurina A, Sakib S, Kerimoglu C, Krüger DM, Kaurani L, Islam R, Centeno TP, Cha J, Xu X, Zeisberg EM, Kranz A, Stewart FA, Fischer A
bioRxiv 2020 : 10.1101/2020.08.07.240853
Postnatal expression of the lysine methyltransferase SETD1B is essential for learning and the regulation of neuron-enriched genes
Michurina A, Sakib MS, Kerimoglu C, Krüger DM, Kaurani L, Islam MR, Joshi PD, Schröder S, Centeno TP, Zhou J, Pradhan R, Cha J, Xu X, Eichele G, Zeisberg EM, Kranz A, Stewart AF, Fischer A
EMBO J 2021 : 10.15252/embj.2020106459
