View primary antibody

Basic data

PIDprimary-0349
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0349
Research groupRG Preobraschenski
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolIGF2
Antibody Registry ID(s)AB_308725
NameAnti-IGF2 antibody
Alternative nameC11orf43 antibody
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withRecombinantFragment
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenHighly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2)
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab9574
Lot no.
DescriptionWB (6) Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Sandwich ELISA Use at an assay dependent concentration. To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of
LocalizationSecreted.
Storage instructionShipped at 4°C. Store at -20ºC.
Receipt date2021-04-17 00:00:00
Preparation date2021-04-17 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

External links

Applications


No application comments yet.

Linked publications


Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2
Kreutzberger AJ, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, Kattan I, Harris M, Liang B, Kenworthy AK, Desai BN, Leitinger N, Anatharam A, Castle JD, Tamm LK
eLife 2020 : 10.7554/elife.62506