View primary antibody

Basic data

PIDprimary-0347
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0347
Research groupRG Preobraschenski
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolVAMP7
Antibody Registry ID(s)
NameAnti-SYBL1/VAMP-7 antibody
Alternative nameFLJ53045 antibody
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withhuman
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenRecombinant fragment corresponding to Human SYBL1/VAMP-7 aa 100-200.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab224535
Lot no.
DescriptionIHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ICC/IF Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
LocalizationCytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Receipt date2021-04-17 00:00:00
Preparation date2021-04-17 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2
Kreutzberger AJ, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, Kattan I, Harris M, Liang B, Kenworthy AK, Desai BN, Leitinger N, Anatharam A, Castle JD, Tamm LK
eLife 2020 : 10.7554/elife.62506