View primary antibody

Basic data

PIDprimary-0345
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0345
Research groupRG Preobraschenski
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolPCSK1/3
Antibody Registry ID(s)
NameRecombinant Anti-PC1/3 antibody
Alternative nameBDP antibody
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withmouse, rat, human
Clone
IsotypeIgG
Clonalitymonoclonal
Demaskingunknown
AntigenRecombinant fragment.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab220363
Lot no.
DescriptionWB 1/1000. Predicted molecular weight: 84 kDa. IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. IHC-Fr 1/500. ICC/IF 1/100. Flow Cyt 1/50. IP 1/30.
LocalizationCytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Receipt date2021-04-17 00:00:00
Preparation date2021-04-17 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2
Kreutzberger AJ, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, Kattan I, Harris M, Liang B, Kenworthy AK, Desai BN, Leitinger N, Anatharam A, Castle JD, Tamm LK
eLife 2020 : 10.7554/elife.62506