Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0269 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0269 |
| Research group | RG Moser |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | OTOF |
| Antibody Registry ID(s) | AB_881807 |
| Name | Anti-Otoferlin antibody |
| Alternative name | AUNB1 antibody |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | mouse, human |
| Clone | 13A9 |
| Isotype | IgG1 |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Tagged fusion protein, corresponding to amino acids 1-395 of Human Otoferlin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab53233 |
| Lot no. | |
| Description | Flow Cyt Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. ICC/IF 1/100 - 1/500. |
| Localization | Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell membrane. Detected at basolateral cell membrane with synaptic vesicles surrounding the ribbon and at the presynaptic plasma membrane in the inner hair cells (IHCs). Colocalizes with GPR25 and RAB8B in inner hair cells. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-04-02 00:00:00 |
| Preparation date | 2021-04-02 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=OTOF |
| Wikipedia | http://en.wikipedia.org/wiki/OTOF |
| Uniprot | http://www.uniprot.org/uniprot/?query=OTOF |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=OTOF |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_881807 |
Applications
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Linked publications
Overloaded Adeno-Associated Virus as a Novel Gene Therapeutic Tool for Otoferlin-Related Deafness
Rankovic V, Vogl C, Dörje NM, Bahader I, Duque-Afonso CJ, Thirumalai A, Weber T, Kusch K, Strenzke N, Moser T
Front Mol Neurosci 2021 : 10.3389/fnmol.2020.600051
A dual‐AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock‐out mice
Al‐Moyed H, Cepeda AP, Jung S, Moser T, Kügler S, Reisinger E
EMBO Mol Med 2018 : 10.15252/emmm.201809396
Probing the role of the C2F domain of otoferlin
Chen H, Fang Q, Benseler F, Brose N, Moser T
Front Mol Neurosci 2023 : 10.3389/fnmol.2023.1299509
