View primary antibody

Basic data

PIDprimary-0178
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0178
Research groupRG Moser
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolNUP107
Antibody Registry ID(s)AB_448181
NameAnti-Nuclear Pore Complex Proteins antibody
Alternative nameNPC proteins antibody
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withmouse, human, SaccharomycesCerevisiae
CloneMab414
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenNuclear pore complex mixture.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab24609
Lot no.
DescriptionICC/IF Use at an assay dependent concentration. Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this
LocalizationNucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-03-09 00:00:00
Preparation date2021-03-09 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Circumvention of common labeling artifacts using secondary nanobodies
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli S, Opazo F
bioRxiv 2019 : 10.1101/818351

Circumvention of common labelling artefacts using secondary nanobodies
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F
Nanoscale 2020 : 10.1039/d0nr00227e