View primary antibody

Basic data

PIDprimary-0103
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0103
Research groupRG Zimmermann
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolCOL1
Antibody Registry ID(s)AB_731684
NameAnti-Collagen I antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withCow, Human
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenFull length native protein (purified) corresponding to Human Collagen I aa 1-1464.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab34710
Lot no.
DescriptionWB 1/1000 - 1/10000. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins here. Customers have been successful using ab34710 in this application, please see references below (Tillgren V et al. J Biol Chem 290:918-25; 2015). Positive Control: Hu stomach, skin and adrenal gland tissue lysates. IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Localization
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
Receipt date2021-01-01 00:00:00
Preparation date2021-01-28 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Inhibition of aquaporin-1 prevents myocardial remodeling by blocking the transmembrane transport of hydrogen peroxide
Montiel V, Bella R, Michel LYM, Esfahani H, De Mulder D, Robinson EL, Deglasse J, Tiburcy M, Chow PH, Jonas J, Gilon P, Steinhorn B, Michel T, Beauloye C, Bertrand L, Farah C, Dei Zotti F, Debaix H, Bouzin C, Brusa D, Horman S, Vanoverschelde J, Bergmann O, Gilis D, Rooman M, Ghigo A, Geninatti-Crich S, Yool A, Zimmermann WH, Roderick HL, Devuyst O, Balligand J
Sci Transl Med 2020 : 10.1126/scitranslmed.aay2176