Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0103 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0103 |
| Research group | RG Zimmermann |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | COL1 |
| Antibody Registry ID(s) | AB_731684 |
| Name | Anti-Collagen I antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | cow, human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Full length native protein (purified) corresponding to Human Collagen I aa 1-1464. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab34710 |
| Lot no. | |
| Description | WB 1/1000 - 1/10000. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins here. Customers have been successful using ab34710 in this application, please see references below (Tillgren V et al. J Biol Chem 290:918-25; 2015). Positive Control: Hu stomach, skin and adrenal gland tissue lysates. IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Localization | |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section. |
| Receipt date | 2021-01-01 00:00:00 |
| Preparation date | 2021-01-28 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=COL1 |
| Wikipedia | http://en.wikipedia.org/wiki/COL1 |
| Uniprot | http://www.uniprot.org/uniprot/?query=COL1 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=COL1 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_731684 |
Applications
No application comments yet.
Linked publications
Inhibition of aquaporin-1 prevents myocardial remodeling by blocking the transmembrane transport of hydrogen peroxide
Montiel V, Bella R, Michel LYM, Esfahani H, De Mulder D, Robinson EL, Deglasse J, Tiburcy M, Chow PH, Jonas J, Gilon P, Steinhorn B, Michel T, Beauloye C, Bertrand L, Farah C, Dei Zotti F, Debaix H, Bouzin C, Brusa D, Horman S, Vanoverschelde J, Bergmann O, Gilis D, Rooman M, Ghigo A, Geninatti-Crich S, Yool A, Zimmermann WH, Roderick HL, Devuyst O, Balligand J
Sci Transl Med 2020 : 10.1126/scitranslmed.aay2176
