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Basic data

Research groupRG Lehnart
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolpan 14-3-3
Antibody Registry ID(s)AB_626618
Namepan 14-3-3 (H-8) antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Human, Avian
Antigenspecific for an epitope mapping between amino acids 1-30 at the N-terminus of 14-3-3 β of human origin
Crafted Bycompany
Company / ManufacturerSanta Cruz Biotechnology
Lot no.
Descriptionpan 14-3-3 (H-8) is recommended for detection of pan 14-3-3 of mouse, ratand human origin by Western Blotting (starting dilution 1:200, dilution range1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500), immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500), flow cytometry (1 μg per 1 x 106cells) and solid phase ELISA (starting dilution 1:30, dilutionrange 1:30-1:3000).
Storage instructionStore at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required
Receipt date2021-01-01 00:00:00
Preparation date2021-01-27 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications

14-3-3 binding creates a memory of kinase action by stabilizing the modified state of phospholamban
Menzel J, Kownatzki-Danger D, Tokar S, Ballone A, Unthan-Fechner K, Kilisch M, Lenz C, Urlaub H, Mori M, Ottmann C, Shattock MJ, Lehnart SE, Schwappach B
Sci Signal 2020 : 10.1126/scisignal.aaz1436

Global kinome profiling reveals DYRK1A as critical activator of the human mitochondrial import machinery
Walter C, Marada A, Suhm T, Ernsberger R, Muders V, Kücükköse C, Sánchez-Martín P, Hu Z, Aich A, Loroch S, Solari FA, Poveda-Huertes D, Schwierzok A, Pommerening H, Matic S, Brix J, Sickmann A, Kraft C, Dengjel J, Dennerlein S, Brummer T, Vögtle F, Meisinger C
Nat Commun 2021 : 10.1038/s41467-021-24426-9

Mammalian oocytes store proteins for the early embryo on cytoplasmic lattices
Jentoft IM, Bäuerlein FJ, Welp LM, Cooper BH, Petrovic A, So C, Penir SM, Politi AZ, Horokhovskyi Y, Takala I, Eckel H, Moltrecht R, Lénárt P, Cavazza T, Liepe J, Brose N, Urlaub H, Fernández-Busnadiego R, Schuh M
Cell 2023 : 10.1016/j.cell.2023.10.003