Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0094 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0094 |
| Research group | RG Lehnart |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | pan 14-3-3 |
| Antibody Registry ID(s) | AB_626618 |
| Name | pan 14-3-3 (H-8) antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | Mouse, Rat, Human, Avian |
| Clone | H-8 |
| Isotype | IgG2b |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | specific for an epitope mapping between amino acids 1-30 at the N-terminus of 14-3-3 β of human origin |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-1657 |
| Lot no. | |
| Description | pan 14-3-3 (H-8) is recommended for detection of pan 14-3-3 of mouse, ratand human origin by Western Blotting (starting dilution 1:200, dilution range1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500), immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500), flow cytometry (1 μg per 1 x 106cells) and solid phase ELISA (starting dilution 1:30, dilutionrange 1:30-1:3000). |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required |
| Receipt date | 2021-01-01 00:00:00 |
| Preparation date | 2021-01-27 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
Applications
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Linked publications
14-3-3 binding creates a memory of kinase action by stabilizing the modified state of phospholamban
Menzel J, Kownatzki-Danger D, Tokar S, Ballone A, Unthan-Fechner K, Kilisch M, Lenz C, Urlaub H, Mori M, Ottmann C, Shattock MJ, Lehnart SE, Schwappach B
Sci Signal 2020 : 10.1126/scisignal.aaz1436
Global kinome profiling reveals DYRK1A as critical activator of the human mitochondrial import machinery
Walter C, Marada A, Suhm T, Ernsberger R, Muders V, Kücükköse C, Sánchez-Martín P, Hu Z, Aich A, Loroch S, Solari FA, Poveda-Huertes D, Schwierzok A, Pommerening H, Matic S, Brix J, Sickmann A, Kraft C, Dengjel J, Dennerlein S, Brummer T, Vögtle F, Meisinger C
Nat Commun 2021 : 10.1038/s41467-021-24426-9
Mammalian oocytes store proteins for the early embryo on cytoplasmic lattices
Jentoft IM, Bäuerlein FJ, Welp LM, Cooper BH, Petrovic A, So C, Penir SM, Politi AZ, Horokhovskyi Y, Takala I, Eckel H, Moltrecht R, Lénárt P, Cavazza T, Liepe J, Brose N, Urlaub H, Fernández-Busnadiego R, Schuh M
Cell 2023 : 10.1016/j.cell.2023.10.003
