| Description |
pan 14-3-3 (H-8) is recommended for detection of pan 14-3-3 of mouse, ratand human origin by Western Blotting (starting dilution 1:200, dilution range1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500), immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500), flow cytometry (1 μg per 1 x 106cells) and solid phase ELISA (starting dilution 1:30, dilutionrange 1:30-1:3000). |