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 External Resources

 218680https://www.addgene.org/218680  Addgene identifier (218680, reused)

 10.17504...v4o/v1https://doi.org/10.17504/protocols.io.5qpvo3mezv4o/v1  DOI (10.17504/protocols.io.5qpvo3mezv4o/v1, protocols.io)

 10.17504/protocols.io.bsgrnbv6https://doi.org/10.17504/protocols.io.bsgrnbv6  DOI (10.17504/protocols.io.bsgrnbv6, protocols.io)

 10.17504...vzp/v1https://doi.org/10.17504/protocols.io.dm6gp3p71vzp/v1  DOI (10.17504/protocols.io.dm6gp3p71vzp/v1, protocols.io)

 10.17504...l8j/v1https://doi.org/10.17504/protocols.io.kxygxyk9dl8j/v1  DOI (10.17504/protocols.io.kxygxyk9dl8j/v1, protocols.io)

 10.17504...lk5/v1https://doi.org/10.17504/protocols.io.n2bvj37kwlk5/v1  DOI (10.17504/protocols.io.n2bvj37kwlk5/v1, protocols.io)

 10.17504...lk5/v1https://doi.org/10.17504/protocols.io.n2bvj3mxxlk5/v1  DOI (10.17504/protocols.io.n2bvj3mxxlk5/v1, protocols.io)

 gro-2/143910http://resolver.sub.uni-goettingen.de/purl?gro-2/143910  GRO.publications identifier

 7108https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=7108  NCBI taxonomy (fall armyworm, Spodoptera frugiperda)

 9606https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606  NCBI taxonomy (human, Homo sapiens)

 4932https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=4932  NCBI taxonomy (yeast, Saccharomyces cerevisiae)

 0000-0003-2782-7318https://orcid.org/0000-0003-2782-7318  ORCID identifier (Olawale G. Raimi)

 0000-0002-8595-5994https://orcid.org/0000-0002-8595-5994  ORCID identifier (Kenneth Ehses)

 0000-0002-6011-8647https://orcid.org/0000-0002-6011-8647  ORCID identifier (Sven M. Lange)

 0009-0006-7557-5388https://orcid.org/0009-0006-7557-5388  ORCID identifier (Verena Dederer)

 0000-0002-1433-4111https://orcid.org/0000-0002-1433-4111  ORCID identifier (Cristian Polo Rivera)

 0000-0002-1337-0612https://orcid.org/0000-0002-1337-0612  ORCID identifier (Rachel Toth)

 0000-0001-8861-379Xhttps://orcid.org/0000-0001-8861-379X  ORCID identifier (Karim P. M. Labib)

 0000-0001-8500-4569https://orcid.org/0000-0001-8500-4569  ORCID identifier (Sebastian Mathea)

 0000-0002-3640-5275https://orcid.org/0000-0002-3640-5275  ORCID identifier (Neil A. Ranson)

 0000-0002-8366-7622https://orcid.org/0000-0002-8366-7622  ORCID identifier (Ruben Fernandez-Busnadiego)

 0000-0001-9733-2404https://orcid.org/0000-0001-9733-2404  ORCID identifier (Miratul M. K. Muqit)

 1010https://prod.mbexc.uni-goettingen.de/literature/publications/1010  Published Data Registry entry (MBExC, Live Cell Imaging Protocol)

 917https://prod.mbexc.uni-goettingen.de/literature/publications/917  Published Data Registry entry (MBExC, preprint)

 1/463https://mbexc.uni-goettingen.de/archive/1/463  Research Data Archive (supplemental material)

 03zj4c476https://ror.org/03zj4c476  ROR identifier (03zj4c476, Aligning Science Across Parkinson's)

 04cvxnb49https://ror.org/04cvxnb49  ROR identifier (04cvxnb49, Goethe University Frankfurt)

 05xy1nn52https://ror.org/05xy1nn52  ROR identifier (05xy1nn52, Multiscale Bioimaging)

 021ft0n22https://ror.org/021ft0n22  ROR identifier (021ft0n22, University Medical Center Göttingen)

 03h2bxq36https://ror.org/03h2bxq36  ROR identifier (03h2bxq36, University of Dundee)

 01nrxwf90https://ror.org/01nrxwf90  ROR identifier (01nrxwf90, University of Edinburgh)

 01y9bpm73https://ror.org/01y9bpm73  ROR identifier (01y9bpm73, University of Göttingen)

 024mrxd33https://ror.org/024mrxd33  ROR identifier (024mrxd33, University of Leeds)

 009kr6r15https://ror.org/009kr6r15  ROR identifier (009kr6r15, Western General Hospital)

 CVCL_0030https://scicrunch.org/resolver/CVCL_0030  SciCrunch identifier (RRID:CVCL_0030, HeLa)

 CVCL_D5JIhttps://scicrunch.org/resolver/CVCL_D5JI  SciCrunch identifier (RRID:CVCL_D5JI, HeLa Flp-In T-REx PINK1 KO clone 3C9)

 SCR_023662https://scicrunch.org/resolver/SCR_023662  SciCrunch identifier (RRID:SCR_023662, AlphaFold Protein Structure Database)

 SCR_016151https://scicrunch.org/resolver/SCR_016151  SciCrunch identifier (RRID:SCR_016151, AMBER)

 SCR_013673https://scicrunch.org/resolver/SCR_013673  SciCrunch identifier (RRID:SCR_013673, Leica Application Suite X)

 SCR_023794https://scicrunch.org/resolver/SCR_023794  SciCrunch identifier (RRID:SCR_023794, Leica Thunder Imager Live Cell system)

 SCR_000305https://scicrunch.org/resolver/SCR_000305  SciCrunch identifier (RRID:SCR_000305, PyMOL)

 science....sm.pdfhttps://www.science.org/doi/suppl/10.1126/sciadv.adn7191/suppl_file/sciadv.adn7191_sm.pdf  Supplemental material

 science....s4.ziphttps://www.science.org/doi/suppl/10.1126/sciadv.adn7191/suppl_file/sciadv.adn7191_tables_s1_to_s4.zip  Supplemental material (table)

 science....s1.ziphttps://www.science.org/doi/suppl/10.1126/sciadv.adn7191/suppl_file/sciadv.adn7191_movie_s1.zip  Supplemental material (video)

 mbexc.de...ystem/https://mbexc.de/mechanism-of-human-pink1-activation-at-the-tom-complex-in-a-reconstituted-system/  Website entry (mbexc.de)

 10.5281/zenodo.10792792https://doi.org/10.5281/zenodo.10792792  Zenodo identifier (10.5281/zenodo.10792792)

 10.5281/zenodo.10829882https://doi.org/10.5281/zenodo.10829882  Zenodo identifier (10.5281/zenodo.10829882)

 10.5281/zenodo.11060950https://doi.org/10.5281/zenodo.11060950  Zenodo identifier (10.5281/zenodo.11060950)

 10.5281/zenodo.11065259https://doi.org/10.5281/zenodo.11065259  Zenodo identifier (10.5281/zenodo.11065259)

 10.5281/zenodo.11065277https://doi.org/10.5281/zenodo.11065277  Zenodo identifier (10.5281/zenodo.11065277)