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Publication
PropertyValue
Working Groups RG Urlaub
SubprojectNone
Open AccessOpen Access Yes
Publication TypeJournal Article
Peer ReviewedYes
PMIDPubMed ID 38467629
DOIDOI 10.1038/s41467-024-46371-z
Publication Year2024
TitleThe thymocyte-specific RNA-binding protein Arpp21 provides TCR repertoire diversity by binding to the 3’-UTR and promoting Rag1 mRNA expression
JournalNature Communications
ISSN2041-1723
eISSN2041-1723
URL http://dx.doi.org/10.1038/s41467-024-46371-z
Issue1
Volume15
Journal AbbreviationNat Commun
AuthorsXu M, Ito-Kureha T, Kang H, Chernev A, Raj T, Hoefig KP, Hohn C, Giesert F, Wang Y, Pan W, Ziętara N, Straub T, Feederle R, Daniel C, Adler B, König J, Feske S, Tsokos GC, Wurst W, Urlaub H, Sattler M, Kisielow J, Wulczyn FG, Łyszkiewicz M, Heissmeyer V
First AuthorXu M
Last AuthorHeissmeyer V

 External Resources

 nature.c...-z.pdf  Article fulltext

 gencodeg...5.html  Genecode (release M25)

 gro-2/142385  GRO.publications identifier

 MSV000091606  MassIVE identifier (MSV000091606, DOI:10.25345/C5WM1440M)

 3038275  MGI identifier (MGI:3038275)

 5544760  MGI identifier (MGI:5544760)

 5883293  MGI identifier (MGI:5883293)

 3812419  MGI identifier (MGI:3812419)

 ncbi.nlm...35.26/  NCBI Datasets Genome (GRCm38.p6, reused)

 GSE198798  NCBI GEO identifier (GSE198798)

 GSE226368  NCBI GEO identifier (GSE226368)

 GSE226369  NCBI GEO identifier (GSE226369)

 NCBI:txid469008  NCBI taxonomy identifier (NCBI:txid469008, E. coli BL21(DE3))

  0000-0003-3895-6422  ORCID identifier (Meng Xu)

 0000-0002-6239-9227  ORCID identifier (Taku Kureha)

 0000-0003-4029-5096  ORCID identifier (Hyun-Seo Kang)

 0000-0002-5065-8092  ORCID identifier (Timsse Raj)

 0000-0002-0701-454X  ORCID identifier (Kai Hoefig)

 0000-0001-6825-8858  ORCID identifier (Florian Giesert)

 0000-0002-3637-8852  ORCID identifier (Yin-Hu Wang)

 0009-0003-5965-2126  ORCID identifier (Wenliang Pan)

 0000-0002-4597-6731  ORCID identifier (Natalia Ziętara)

 0000-0002-0547-0453  ORCID identifier (Tobias Straub)

 0000-0002-3981-367X  ORCID identifier (Regina Feederle)

 0000-0003-4698-7069  ORCID identifier (Carolin Daniel)

 0000-0002-9398-5421  ORCID identifier (Julian König)

 0000-0001-5431-8178  ORCID identifier (Stefan Feske)

 0000-0001-9589-2360  ORCID identifier (George Tsokos)

 0000-0003-4422-7410  ORCID identifier (Wolfgang Wurst)

 0000-0003-1837-5233  ORCID identifier (Henning Urlaub)

 0000-0002-1594-0527  ORCID identifier (Michael Sattler)

 0000-0002-4482-5202  ORCID identifier (Jan Kisielow)

 0000-0001-9850-2444  ORCID identifier (F. Gregory Wulczyn)

 0000-0002-2263-8545  ORCID identifier (Vigo Heissmeyer)

 04drvxt59  ROR identifier (04drvxt59, Beth Israel Deaconess Medical Center)

 0493xsw21  ROR identifier (0493xsw21, Berlin Institute of Health at Charité - Universitätsmedizin Berlin)

 05a28rw58  ROR identifier (05a28rw58, ETH Zurich)

 04qq88z54  ROR identifier (04qq88z54, German Center for Diabetes Research)

 043j0f473  ROR identifier (043j0f473, German Center for Neurodegenerative Diseases)

 00cfam450  ROR identifier (00cfam450, Helmholtz Zentrum München)

 05kxtq558  ROR identifier (05kxtq558, Institute of Molecular Biology)

 05591te55  ROR identifier (05591te55, Ludwig-Maximilians-Universität München)

 03av75f26  ROR identifier (03av75f26, Max Planck Institute for Multidisciplinary Sciences)

 05xy1nn52  ROR identifier (05xy1nn52, Multiscale Bioimaging)

 025z3z560  ROR identifier (025z3z560, Munich Cluster of Systems Neurology)

 0190ak572  ROR identifier (0190ak572, New York University)

 02kkvpp62  ROR identifier (02kkvpp62, Technical University of Munich)

 01y9bpm73  ROR identifier (01y9bpm73, University of Göttingen)

 021ft0n22  ROR identifier (021ft0n22, University Medical Center Göttingen)

 05emabm63  ROR identifier (05emabm63, University Medical Center Ulm)

 0371fqr87  ROR identifier (0371fqr87, Wuhan Union Hospital)

 Addgene_12371  SciCrunch identifier (RRID:Addgene_12371, pCL-Eco)

 Addgene_17442  SciCrunch identifier (RRID:Addgene_17442, MSCV-IRES-Thy1.1)

 AB_2877798  SciCrunch identifier (RRID:AB_2877798, Arpp21)

 AB_2876392  SciCrunch identifier (RRID:AB_2876392, CD3 APC-Fire 810)

 AB_2870246  SciCrunch identifier (RRID:AB_2870246, CD4 BUV661)

 AB_10898318  SciCrunch identifier (RRID:AB_10898318, CD4 BV421)

 AB_2564587  SciCrunch identifier (RRID:AB_2564587, CD4 BV510)

 AB_2728707  SciCrunch identifier (RRID:AB_2728707, CD4 BV786)

 AB_11124344  SciCrunch identifier (RRID:AB_11124344, CD8 BV650)

 AB_2871881  SciCrunch identifier (RRID:AB_2871881, CD8 BV750)

 AB_464915  SciCrunch identifier (RRID:AB_464915, CD8 FITC)

 AB_2895935  SciCrunch identifier (RRID:AB_2895935, CD11c BUV737)

 AB_2739519  SciCrunch identifier (RRID:AB_2739519, CD11b BV480)

 AB_464940  SciCrunch identifier (RRID:AB_464940, CD11c FITC)

 AB_2565970  SciCrunch identifier (RRID:AB_2565970, CD19 BV711)

 AB_2563464  SciCrunch identifier (RRID:AB_2563464, CD24 BV605)

 AB_1834425  SciCrunch identifier (RRID:AB_1834425, CD24 PerCP-eFluor 710)

 AB_763472  SciCrunch identifier (RRID:AB_763472, CD25 AF488)

 AB_395101  SciCrunch identifier (RRID:AB_395101, CD25 PE)

 AB_2616762  SciCrunch identifier (RRID:AB_2616762, CD25 PE-Cy7)

 AB_914324  SciCrunch identifier (RRID:AB_914324, CD25 PerCP-Cy5.5)

 AB_2869776  SciCrunch identifier (RRID:AB_2869776, CD28 BB700)

 AB_312871  SciCrunch identifier (RRID:AB_312871, CD28 PE)

 AB_528811  SciCrunch identifier (RRID:AB_528811, CD43 PE-Cy5)

 AB_10895752  SciCrunch identifier (RRID:AB_10895752, CD44 BV421)

 AB_2561391  SciCrunch identifier (RRID:AB_2561391, CD44 BV510)

 AB_10900641  SciCrunch identifier (RRID:AB_10900641, CD44 BV570)

 AB_11203907  SciCrunch identifier (RRID:AB_11203907, CD45R/B220 BV605)

 AB_469627  SciCrunch identifier (RRID:AB_469627, CD45R/B220 PE-Cy7)

 AB_1963590  SciCrunch identifier (RRID:AB_1963590, CD62L eF450)

 AB_493564  SciCrunch identifier (RRID:AB_493564, CD69 PE-Cy7)

 AB_398536  SciCrunch identifier (RRID:AB_398536, CD117 APC)

 AB_2800691  SciCrunch identifier (RRID:AB_2800691, CD117 BV421)

 AB_2895995  SciCrunch identifier (RRID:AB_2895995, CD127 BUV737)

 AB_2107426  SciCrunch identifier (RRID:AB_2107426, Gapdh)

 AB_906247  SciCrunch identifier (RRID:AB_906247, Gr1 (Ly-6G/Ly-6C) PerCP-Cy5.5)

 AB_2662805  SciCrunch identifier (RRID:AB_2662805, Gr1 (Ly-6G/Ly-6C) SB645)

 AB_10660749  SciCrunch identifier (RRID:AB_10660749, Helios PE)

 AB_330924  SciCrunch identifier (RRID:AB_330924, HRP α-Mouse)

 AB_2099233  SciCrunch identifier (RRID:AB_2099233, HRP α-Rabbit)

 AB_10694715  SciCrunch identifier (RRID:AB_10694715, HRP α-Rat)

 AB_2571985  SciCrunch identifier (RRID:AB_2571985, IgD PE-Dazzle 594)

 AB_2875475  SciCrunch identifier (RRID:AB_2875475, IgM BUV615)

 AB_10899576  SciCrunch identifier (RRID:AB_10899576, IgM BV421)

 AB_2338618  SciCrunch identifier (RRID:AB_2338618, IgM FITC)

 AB_1272204  SciCrunch identifier (RRID:AB_1272204, MHC Class II (I-Ab) eFluor450)

 AB_2870785  SciCrunch identifier (RRID:AB_2870785, NK1.1 (CD161) BUV563)

 AB_10734230  SciCrunch identifier (RRID:AB_10734230, PAX5 APC)

 AB_2629684  SciCrunch identifier (RRID:AB_2629684, Sca1 (Ly-6A/E) BV650)

 AB_313425  SciCrunch identifier (RRID:AB_313425, TCRβ)

 AB_2740818  SciCrunch identifier (RRID:AB_2740818, TCRβ BUV395)

 AB_893627  SciCrunch identifier (RRID:AB_893627, TCRβ PE-Cy7)

 AB_1731813  SciCrunch identifier (RRID:AB_1731813, TCRγδ APC)

 AB_2744919  SciCrunch identifier (RRID:AB_2744919, TCRγδ SB780)

 AB_2870685  SciCrunch identifier (RRID:AB_2870685, Ter-119 BUV496)

 AB_628410  SciCrunch identifier (RRID:AB_628410, Tubulin)

 SCR_018043  SciCrunch identifier (RRID:SCR_018043, Bioanalyzer 2100)

 SCR_019826  SciCrunch identifier (RRID:SCR_019826, CYTEK Aurora)

 SCR_016695  SciCrunch identifier (RRID:SCR_016695, FACS Aria III BD)

 SCR_014583  SciCrunch identifier (RRID:SCR_014583, FastQC)

 SCR_008520  SciCrunch identifier (RRID:SCR_008520, FlowJo)

 SCR_000306  SciCrunch identifier (RRID:SCR_000306, GraphPad)

 SCR_010881  SciCrunch identifier (RRID:SCR_010881, Homer)

 SCR_018006  SciCrunch identifier (RRID:SCR_018006, Illumina HiSeq 1500)

 SCR_016379  SciCrunch identifier (RRID:SCR_016379, Illumina MiSeq)

 SCR_016582  SciCrunch identifier (RRID:SCR_016582, kallisto)

 SCR_018626  SciCrunch identifier (RRID:SCR_018626, LightCycler 480)

 SCR_014485  SciCrunch identifier (RRID:SCR_014485, MaxQuant software)

 SCR_018725  SciCrunch identifier (RRID:SCR_018725, MIXCR)

 SCR_023795  SciCrunch identifier (RRID:SCR_023795, MicroCal PEAQ-ITC)

 SCR_012042  SciCrunch identifier (RRID:SCR_012042, OpenMS)

 SCR_020573  SciCrunch identifier (RRID:SCR_020573, Orbitrap Fusion Tribrid)

 SCR_015753  SciCrunch identifier (RRID:SCR_015753, Perseus)

 SCR_020553  SciCrunch identifier (RRID:SCR_020553, Qubit)

 SCR_014228  SciCrunch identifier (RRID:SCR_014228, Sparky)

 SCR_004463  SciCrunch identifier (RRID:SCR_004463, STAR)

 SCR_014227  SciCrunch identifier (RRID:SCR_014227, TopSpin)

 UP000000589  UniProt Proteome identifier (UP000000589, reused)

 static-c...SM.pdf  Supplemental material (Supplementary Information)

 static-c...SM.pdf  Supplemental material (Peer Review File)

 static-c...SM.pdf  Supplemental material (Description of Additional Supplementary Files)

 static-c...M.xlsx  Supplemental material (Supplementary Data 1)

 static-c...SM.pdf  Supplemental material (Reporting Summary)

 static-c...M.xlsx  Supplemental material (Source data)

 mbexc.de...ssion/  Website entry (mbexc.de)

Linked Antibodies

PIDAGTypeAntigen symbolAntibody Registry IDName
primary-1477primaryARPP-21AB_2877798ARPP-21 antibody
primary-1478primaryCD3AB_2876392CD3 APC-Fire 810
primary-1479primaryCD4AB_2870246CD4 BUV661
primary-1480primaryCD4AB_10898318Brilliant Violet 421™ anti-mouse CD4 Antibody
primary-1481primaryCD4AB_2564587Brilliant Violet 510™ anti-mouse CD4 Antibody
primary-1482primaryCD4AB_2728707BV786 Rat Anti-Mouse CD4
primary-1483primaryCD8AB_11124344Brilliant Violet 650™ anti-mouse CD8a Antibody
primary-1484primaryCD8AB_2871881CD8A BV750
primary-1485primaryCD8AB_464915CD8a Monoclonal Antibody FITC
primary-1486primaryCD11AB_2895935CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet™ 737
primary-1487primaryCD11bAB_2739519BD Horizon™ BV480 Rat Anti-CD11b
primary-1488primaryCD11cAB_464940CD11c Monoclonal Antibody (N418), FITC
primary-1489primaryCD19AB_2565970Brilliant Violet 711™ anti-mouse CD19 Antibody
primary-1490primaryCD24AB_2563464Brilliant Violet 605™ anti-mouse CD24 Antibody
primary-1491primaryCD24AB_1834425CD24 Monoclonal Antibody (M1/69), PerCP-eFluor™ 710
primary-1492primaryCD25AB_763472CD25 Monoclonal Antibody (PC61.5), Alexa Fluor™ 488
primary-1493primaryCD25AB_395101PE Rat Anti-Mouse CD25
primary-1494primaryCD25AB_2616762PE/Cyanine7 anti-mouse CD25 Antibody
primary-1495primaryCD25AB_914324CD25 Monoclonal Antibody (PC61.5), PerCP-Cyanine5.5
primary-1496primaryCD28AB_2869776BB700 Hamster Anti-Mouse CD28
primary-1497primaryCD28AB_312871PE anti-mouse CD28 Antibody
primary-1498primaryCD43AB_528811PE/Cyanine5 anti-mouse CD43 Activation-Associated Glycoform Antibody
primary-1499primaryCD44AB_10895752Brilliant Violet 421™ anti-mouse/human CD44 Antibody
primary-1500primaryCD44AB_2561391Brilliant Violet 510™ anti-mouse/human CD44 Antibody
primary-1501primaryCD44AB_10900641Brilliant Violet 570™ anti-mouse/human CD44 Antibody
primary-1502primaryCD45RAB_11203907Brilliant Violet 605™ anti-mouse/human CD45R/B220 Antibody
primary-1503primaryCD45RAB_469627CD45R (B220) Monoclonal Antibody (RA3-6B2), PE-Cyanine7
primary-1504primaryCD62LAB_1963590CD62L (L-Selectin) Monoclonal Antibody (MEL-14), eFluor™ 450
primary-1505primaryCD69AB_493564PE/Cyanine7 anti-mouse CD69 Antibody
primary-1506primaryCD117AB_398536APC Rat Anti-Mouse CD117
primary-1507primaryCD117AB_2800691Brilliant Violet 421™ anti-mouse CD117 (c-kit) Recombinant Antibody
primary-1508primaryCD127AB_2895995CD127 Monoclonal Antibody (A7R34), Brilliant Ultra Violet™ 737
primary-1509primaryGADPHAB_2107426Anti-GAPDH Mouse mAb (6C5)
primary-1510primaryAB_906247Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PerCP-Cyanine5.5
primary-1511primaryAB_2662805Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Super Bright™ 645
primary-1512primaryIKZF2AB_10660749PE anti-mouse/human Helios
primary-1516primaryIgDAB_2571985PE/Dazzle™ 594 anti-mouse IgD Antibody
primary-1517primaryIgMAB_2875475BUV615 Rat Anti-Mouse IgM
primary-1518primaryIgMAB_10899576Brilliant Violet 421™ anti-mouse IgM Antibody
primary-1519primaryIgMAB_2338618Fluorescein (FITC)-AffiniPure Fab Fragment Goat Anti-Mouse IgM, µ Chain Specific
primary-1520primaryAB_1272204MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), eFluor™ 450
primary-1521primaryAB_2870785BUV563 Mouse Anti-Mouse NK-1.1
primary-1522primaryPAX5AB_10734230PAX5 Monoclonal Antibody (1H9), APC
primary-1523primarySCA1AB_2629684Brilliant Violet 650™ anti-mouse Ly-6A/E (Sca-1) Antibody
primary-1524primaryTRBAB_313425anti-mouse TCR β chain Antibody
primary-1525primaryTRBAB_2740818BUV395 Hamster Anti-Mouse TCR β Chain
primary-1526primaryTRBAB_893627PE/Cyanine7 anti-mouse TCR β chain Antibody
primary-1527primaryTCRAB_1731813APC Anti-mouse TCR γ/δ Antibody
primary-1528primaryAB_2744919TCRγδ Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Super Bright™ 780
primary-1529primaryTER-119AB_2870685BUV496 Rat Anti-Mouse TER-119/Erythroid Cells
primary-1530primaryTUBAAB_628410alpha Tubulin (B-5-1-2)
secondary-1513secondaryAB_330924Anti-mouse IgG, HRP-linked Antibody
secondary-1514secondaryAB_2099233Anti-rabbit IgG, HRP-linked Antibody
secondary-1515secondaryAB_10694715Anti-rat IgG, HRP-linked Antibody