| Property |
Value |
| Working Groups |
RG Rizzoli, 
RG Simeth |
| Subproject |
None |
| Open Access |
Yes |
| Publication Type |
Journal Article |
| Peer Reviewed |
Yes |
| PMID |
39384781 |
| DOI |
10.1038/s41467-024-53030-w |
| Publication Year |
2024 |
| Title |
NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals |
| Journal |
Nature Communications |
| ISSN |
2041-1723 |
| eISSN |
2041-1723 |
| URL |
http://dx.doi.org/10.1038/s41467-024-53030-w |
| Issue |
1 |
| Volume |
15 |
| Journal Abbreviation |
Nat Commun |
| Authors |
Mougios N, Cotroneo ER, Imse N, Setzke J, Rizzoli SO, Simeth NA, Tsukanov R, Opazo F |
| First Author |
Mougios N |
| Last Author |
Opazo F |
External Resources
nature.c...-w.pdfhttps://www.nature.com/articles/s41467-024-53030-w.pdf Article fulltext
9534https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9534 NCBI taxonomy (African green monkey, Chlorocebus aethiops)
9606https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 NCBI taxonomy (human, Homo sapiens)
10116https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 NCBI taxonomy (rat, Rattus norvegicus)
0009-0007-5712-7175https://orcid.org/0009-0007-5712-7175 ORCID identifier (Nikolaos Mougios)
0009-0000-5614-4378https://orcid.org/0009-0000-5614-4378 ORCID identifier (Jonas Setzke)
0000-0002-1667-7839https://orcid.org/0000-0002-1667-7839 ORCID identifier (Silvio-Olivier Rizzoli)
0000-0001-8130-883Xhttps://orcid.org/0000-0001-8130-883X ORCID identifier (Nadja Anita Simeth-Crespi)
0000-0002-3967-1755https://orcid.org/0000-0002-3967-1755 ORCID identifier (Roman Tsukanov)
0000-0002-4968-9713https://orcid.org/0000-0002-4968-9713 ORCID identifier (Felipe Opazo)
297https://sfb1286.uni-goettingen.de/literature/publications/297 Published Data Registry entry (CRC 1286)
924https://prod.mbexc.uni-goettingen.de/literature/publications/924 Published Data Registry entry (MBExC, preprint)
1/520https://mbexc.uni-goettingen.de/archive/1/520 Research Data Archive (supplemental material)
05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)
021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)
01y9bpm73https://ror.org/01y9bpm73 ROR identifier (01y9bpm73, University of Göttingen)
AB_399853https://scicrunch.org/resolver/AB_399853 SciCrunch identifier (RRID:AB_399853)
AB_628381https://scicrunch.org/resolver/AB_628381 SciCrunch identifier (RRID:AB_628381)
AB_628437https://scicrunch.org/resolver/AB_628437 SciCrunch identifier (RRID:AB_628437)
AB_779033https://scicrunch.org/resolver/AB_779033 SciCrunch identifier (RRID:AB_779033)
AB_887717https://scicrunch.org/resolver/AB_887717 SciCrunch identifier (RRID:AB_887717)
AB_887730https://scicrunch.org/resolver/AB_887730 SciCrunch identifier (RRID:AB_887730)
AB_887770https://scicrunch.org/resolver/AB_887770 SciCrunch identifier (RRID:AB_887770)
AB_887792https://scicrunch.org/resolver/AB_887792 SciCrunch identifier (RRID:AB_887792)
AB_887811https://scicrunch.org/resolver/AB_887811 SciCrunch identifier (RRID:AB_887811)
AB_887832https://scicrunch.org/resolver/AB_887832 SciCrunch identifier (RRID:AB_887832)
AB_887859https://scicrunch.org/resolver/AB_887859 SciCrunch identifier (RRID:AB_887859)
AB_1080447https://scicrunch.org/resolver/AB_1080447 SciCrunch identifier (RRID:AB_1080447)
AB_1849446https://scicrunch.org/resolver/AB_1849446 SciCrunch identifier (RRID:AB_1849446)
AB_1857362https://scicrunch.org/resolver/AB_1857362 SciCrunch identifier (RRID:AB_1857362)
AB_2083165https://scicrunch.org/resolver/AB_2083165 SciCrunch identifier (RRID:AB_2083165)
AB_2147096https://scicrunch.org/resolver/AB_2147096 SciCrunch identifier (RRID:AB_2147096)
AB_2545045https://scicrunch.org/resolver/AB_2545045 SciCrunch identifier (RRID:AB_2545045)
AB_2619773https://scicrunch.org/resolver/AB_2619773 SciCrunch identifier (RRID:AB_2619773)
AB_2619811https://scicrunch.org/resolver/AB_2619811 SciCrunch identifier (RRID:AB_2619811)
AB_2619813https://scicrunch.org/resolver/AB_2619813 SciCrunch identifier (RRID:AB_2619813)
AB_3075906https://scicrunch.org/resolver/AB_3075906 SciCrunch identifier (RRID:AB_3075906)
AB_3075980https://scicrunch.org/resolver/AB_3075980 SciCrunch identifier (RRID:AB_3075980)
AB_3075982https://scicrunch.org/resolver/AB_3075982 SciCrunch identifier (RRID:AB_3075982)
AB_3075983https://scicrunch.org/resolver/AB_3075983 SciCrunch identifier (RRID:AB_3075983)
AB_3075986https://scicrunch.org/resolver/AB_3075986 SciCrunch identifier (RRID:AB_3075986)
AB_3076003https://scicrunch.org/resolver/AB_3076003 SciCrunch identifier (RRID:AB_3076003)
AB_3076005https://scicrunch.org/resolver/AB_3076005 SciCrunch identifier (RRID:AB_3076005)
AB_3076025https://scicrunch.org/resolver/AB_3076025 SciCrunch identifier (RRID:AB_3076025)
AB_3076046https://scicrunch.org/resolver/AB_3076046 SciCrunch identifier (RRID:AB_3076046)
AB_3076057https://scicrunch.org/resolver/AB_3076057 SciCrunch identifier (RRID:AB_3076057)
AB_3076106https://scicrunch.org/resolver/AB_3076106 SciCrunch identifier (RRID:AB_3076106)
AB_3076117https://scicrunch.org/resolver/AB_3076117 SciCrunch identifier (RRID:AB_3076117)
AB_3076136https://scicrunch.org/resolver/AB_3076136 SciCrunch identifier (RRID:AB_3076136)
AB_10672335https://scicrunch.org/resolver/AB_10672335 SciCrunch identifier (RRID:AB_10672335)
AB_11042616https://scicrunch.org/resolver/AB_11042616 SciCrunch identifier (RRID:AB_11042616)
AB_11064600https://scicrunch.org/resolver/AB_11064600 SciCrunch identifier (RRID:AB_11064600)
AB_11181058https://scicrunch.org/resolver/AB_11181058 SciCrunch identifier (RRID:AB_11181058)
CVCL_0224https://scicrunch.org/resolver/CVCL_0224 SciCrunch identifier (RRID:CVCL_0224, COS-7)
CVCL_0042https://scicrunch.org/resolver/CVCL_0042 SciCrunch identifier (RRID:CVCL_0042, U2OS)
RGD_13508588https://scicrunch.org/resolver/RGD_13508588 SciCrunch identifier (RRID:RGD_13508588, WI)
SCR_002285https://scicrunch.org/resolver/SCR_002285 SciCrunch identifier (RRID:SCR_002285, Fiji)
SCR_002798https://scicrunch.org/resolver/SCR_002798 SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)
SCR_003070https://scicrunch.org/resolver/SCR_003070 SciCrunch identifier (RRID:SCR_003070, ImageJ)
static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM1_ESM.pdf Supplemental material
goeseek.gwdguser.de/studies/205https://goeseek.gwdguser.de/studies/205 Supplemental material (goeseek study)
static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM3_ESM.pdf Supplemental material (peer review)
static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM2_ESM.pdf Supplemental material (reporting summary)
static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM4_ESM.xlsx Supplemental material (source data)
mbexc.de...gnals/https://mbexc.de/nanoplex-a-universal-strategy-for-fluorescence-microscopy-multiplexing-using-nanobodies-with-erasable-signals/ Website entry (mbexc.de)
Linked Antibodies
| PID |
AG |
Type |
Antigen symbol |
Antibody Registry ID |
Name |
| primary-0130 |
|
primary |
DOC2A/DOC2B |
AB_11064600 |
Anti-Doc 2a/b antibody |
| primary-0151 |
 |
primary |
VAMP2 |
AB_887811 |
Anti-Synaptobrevin 2 antibody |
| primary-0257 |
 |
primary |
CLTC |
AB_2083165 |
Anti-Clathrin heavy chain antibody |
| primary-0335 |
 |
primary |
SYT1 |
AB_887832 |
Anti-Synaptotagmin 1 antibody |
| primary-0553 |
|
primary |
HOMER1 |
AB_887730 |
Anti-Homer 1 antibody |
| primary-0636 |
 |
primary |
BSN |
AB_11181058 |
Bassoon monoclonal antibody |
| primary-0691 |
|
primary |
SPTBN2 |
AB_399853 |
Purified Mouse Anti-β-Spectrin II |
| primary-1435 |
 |
primary |
Rab3a |
AB_887770 |
Rab3a antibody |
| primary-1470 |
 |
primary |
Tom20 |
AB_628381 |
Tom20 Antikörper (F-10) |
| primary-1726 |
|
primary |
SNCA/SNCB |
AB_2619811 |
alpha Synuclein antibody |
| primary-1734 |
|
primary |
VIM |
AB_628437 |
Vimentin Antibody (V9) |
| primary-1735 |
|
primary |
|
AB_779033 |
Anti-Paxillin antibody [Y113] |
| primary-1736 |
|
primary |
GEPH |
AB_887717 |
Gephyrin antibody |
| primary-1737 |
|
primary |
SNAP25 |
AB_887792 |
SNAP25 antibody |
| primary-1738 |
|
primary |
|
AB_887859 |
alpha-Tubulin antibody |
| primary-1739 |
|
primary |
UBTF |
AB_1080447 |
Anti-UBTF antibody produced in rabbit |
| primary-1740 |
|
primary |
GALNT2 |
AB_1849446 |
Anti-GALNT2 antibody produced in rabbit |
| primary-1741 |
|
primary |
SON |
AB_1857362 |
Anti-SON antibody produced in rabbit |
| primary-1742 |
|
primary |
MAP2 |
AB_2147096 |
MAP2 antibody |
| primary-1743 |
|
primary |
VGAT |
AB_2545045 |
VGAT Polyclonal Antibody |
| primary-1744 |
|
primary |
SYN1 |
AB_2619773 |
Synapsin1/2 antibody |
| primary-1745 |
|
primary |
NLGN2 |
AB_2619813 |
Neuroligin2 antibody |
| primary-1746 |
|
primary |
GFP |
AB_3075906 |
FluoTag®-X4 anti-GFP Atto643 |
| primary-1747 |
|
primary |
ALFA |
AB_3075980 |
FluoTag®-X2 anti-ALFA AZDye568 |
| primary-1748 |
|
primary |
ALFA |
AB_3075982 |
FluoTag®-X2 anti-ALFA Atto488 |
| primary-1749 |
|
primary |
ALFA |
AB_3075983 |
FluoTag®-X2 anti-ALFA Atto643 |
| primary-1750 |
|
primary |
ALFA |
AB_3075986 |
sdAb anti-ALFA unconjugated |
| primary-1751 |
|
primary |
VGlut1 |
AB_3076003 |
FluoTag®-X2 anti-VGlut1 AZDye568 |
| primary-1752 |
|
primary |
VGlut1 |
AB_3076005 |
FluoTag®-X2 anti-VGlut1 Atto488 |
| primary-1753 |
|
primary |
Mouse IgG1 |
AB_3076025 |
sdAb anti-Mouse IgG1 unconjugated |
| primary-1754 |
|
primary |
Rabbit IgG |
AB_3076046 |
sdAb anti-Rabbit IgG unconjugated |
| primary-1755 |
|
primary |
Mouse IgG2a/b |
AB_3076057 |
sdAb anti-Mouse IgG2a/b unconjugated |
| primary-1756 |
|
primary |
PSD95 |
AB_3076106 |
FluoTag®-X2 anti-PSD95 Atto643 |
| primary-1757 |
|
primary |
GFAP |
AB_3076117 |
FluoTag®-X2 anti-GFAP Atto488 |
| primary-1758 |
|
primary |
SYT1 |
AB_3076136 |
FluoTag®-X2 anti-Syt1cyt (A51) AZDye568 |
| primary-1759 |
|
primary |
PMP70 |
|
Anti-PMP70 antibody |
| primary-1760 |
|
primary |
SV2B |
AB_11042616 |
SV2 B antibody |