| Antigen symbol |
TSG101 |
| Antibody Registry ID(s) |
AB_2208084 |
| Name |
Anti-TSG101 antibody |
| Alternative name |
VPS23 |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
mouse, rat, human |
| Clone |
|
| Isotype |
IgG |
| Clonality |
polyclonal |
| Demasking |
unknown |
| Antigen |
Synthetic peptide corresponding to Human TSG101 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab30871 |
| Lot no. |
|
| Description |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48,49 kDa (predicted molecular weight: 44 kDa).Can be blocked with TSG101 peptide (ab30870).
The doublet seen in Western blot has been described in PMID:11427703 and may be due to internal initiation at Met10.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| Localization |
Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. |
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date |
2022-02-26 00:00:00 |
| Preparation date |
2022-02-26 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
Klauenberg, Vanessa |