View primary antibody

Basic data

PID primary-0405
EPIC PID
Research group RG Wollnik
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol NCAM1
Antibody Registry ID(s) AB_528389
Name Anti-NCAM Antibody
Alternative name CD56
Lab ID
Tag / Fluorophore
Raised in
Reacts with avian, chicken, frog, Xenopus
Clone
Isotype MIgG1
Clonality monoclonal
Demasking unknown
Antigen NCAM (cytoplasmic domain), 180 kDa isoform
Crafted By company
Company / Manufacturer DSHB
Catalog no. 4d
Lot no.
Description Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Localization
Storage instruction Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Receipt date 2021-04-23 00:00:00
Preparation date 2021-04-23 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

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Linked publications


De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome
Ufartes R et al.
Hum Genet 2020: 10.1007/s00439-020-02175-x