View primary antibody

Basic data

PID primary-0404
EPIC PID
Research group RG Wollnik
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol COL2A1
Antibody Registry ID(s) AB_528165
Name Anti- collagen type II Antibody
Alternative name collagen, type II, alpha 1
Lab ID
Tag / Fluorophore
Raised in
Reacts with chicken, avian, bovine, fish, goat, human, mouse, Ovine, Quail, rabbit, rat, shark, Xenopus, zebrafish
Clone
Isotype MIgG1
Clonality monoclonal
Demasking unknown
Antigen Collagen type II, Full length protein
Crafted By company
Company / Manufacturer DSHB
Catalog no. II-II6B3
Lot no.
Description Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Localization
Storage instruction Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Receipt date 2021-04-23 00:00:00
Preparation date 2021-04-23 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

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Linked publications


De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome
Ufartes R et al.
Hum Genet 2020: 10.1007/s00439-020-02175-x