| Antigen symbol |
NES |
| Antibody Registry ID(s) |
AB_305313 |
| Name |
Anti-Nestin antibody |
| Alternative name |
ESTM 46 antibody |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
mouse, rat, human |
| Clone |
Rat-401 |
| Isotype |
IgG1 |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
Tissue, cells or virus corresponding to Rat Nestin. Homogenized spinal cord tissue from embryonic day 15 (E15) rats. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab6142 |
| Lot no. |
|
| Description |
IHC-P Use a concentration of 0.05 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 200 kDa. Block with milk or BSA but do not dilute primary antibody in buffer containing milk. |
| Localization |
|
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Receipt date |
2021-04-06 00:00:00 |
| Preparation date |
2021-04-06 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
Klauenberg, Vanessa |