View primary antibody

Basic data

PIDprimary-1122
EPIC PIDhttps://hdl.handle.net/21.11124/primary-1122
Research groupRG Fischer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolGFAP
Antibody Registry ID(s)AB_177521
NameAnti-Glial Fibrillary Acidic Protein Antibody
Alternative nameFLJ45472
Lab ID
Tag / Fluorophore
Raised inChicken
Reacts withHuman, Mouse, Rat, Bovine, Pig
Clone
Isotype
Clonalitypolyclonal
Demaskingunknown
AntigenPurified bovine GFAP.
Crafted Bycompany
Company / ManufacturerMerck Millipore
Catalog no.AB5541
Lot no.
DescriptionImmunohistochemistry(paraffin): Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain. Immunocytochemistry: 1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol. Western blot: 1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa. Immunohistochemistry: 1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde. Optimal working dilutions must be determined by the end user.
Localization
Storage instructionStable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.
Receipt date2022-09-08 00:00:00
Preparation date2022-09-08 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3
Baidoe-Ansah D, Sakib S, Jia S, Mirzapourdelavar H, Strackeljan L, Fischer A, Aleshin S, Kaushik R, Dityatev A
Cells 2022 : 10.3390/cells11132033