View primary antibody

Basic data

PIDprimary-0656
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0656
Research groupRG Brose
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolGFP
Antibody Registry ID(s)AB_94936
NameAnti-Green Fluorescent Protein Antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withall
Clone
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenGFP-BSA
Crafted Bycompany
Company / ManufacturerMerck Millipore
Catalog no.MAB3580
Lot no.
DescriptionWestern blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA. Immunocytochemistry: 1:500-1:1,000 Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA. ELISA: 1:1,000-1:2,000 in a direct assay. Optimal working dilutions must be determined by the end user.
Localization
Storage instructionMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Receipt date2021-08-07 00:00:00
Preparation date2021-08-07 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


A fluorescent nanosensor paint reveals the heterogeneity of dopamine release from neurons at individual release sites
Elizarova S, Chouaib A, Shaib A, Mann F, Brose N, Kruss S, Daniel J
bioRxiv 2021 : 10.1101/2021.03.28.437019

A fluorescent nanosensor paint detects dopamine release at axonal varicosities with high spatiotemporal resolution
Elizarova S, Chouaib AA, Shaib A, Hill B, Mann F, Brose N, Kruss S, Daniel JA
Proc Natl Acad Sci U S A 2022 : 10.1073/pnas.2202842119