Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
PID | primary-0656 |
EPIC PID | https://hdl.handle.net/21.11124/primary-0656 |
Research group | RG Brose |
Quality (mean) | no score (0.00) |
Sharing level | Public |
Antigen symbol | GFP |
Antibody Registry ID(s) | AB_94936 |
Name | Anti-Green Fluorescent Protein Antibody |
Alternative name | |
Lab ID | |
Tag / Fluorophore | |
Raised in | Mouse |
Reacts with | all |
Clone | |
Isotype | IgG1 |
Clonality | monoclonal |
Demasking | unknown |
Antigen | GFP-BSA |
Crafted By | company |
Company / Manufacturer | Merck Millipore |
Catalog no. | MAB3580 |
Lot no. | |
Description | Western blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA. Immunocytochemistry: 1:500-1:1,000 Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA. ELISA: 1:1,000-1:2,000 in a direct assay. Optimal working dilutions must be determined by the end user. |
Localization | |
Storage instruction | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Receipt date | 2021-08-07 00:00:00 |
Preparation date | 2021-08-07 00:00:00 |
Created by | Klauenberg, Vanessa |
Last modified | Klauenberg, Vanessa |
Antibodypedia | - |
HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=GFP |
Wikipedia | http://en.wikipedia.org/wiki/GFP |
Uniprot | http://www.uniprot.org/uniprot/?query=GFP |
GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=GFP |
Antibody Registry | http://antibodyregistry.org/search?q=AB_94936 |
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A fluorescent nanosensor paint reveals the heterogeneity of dopamine release from neurons at individual release sites
Elizarova S, Chouaib A, Shaib A, Mann F, Brose N, Kruss S, Daniel J
bioRxiv 2021 : 10.1101/2021.03.28.437019
A fluorescent nanosensor paint detects dopamine release at axonal varicosities with high spatiotemporal resolution
Elizarova S, Chouaib AA, Shaib A, Hill B, Mann F, Brose N, Kruss S, Daniel JA
Proc Natl Acad Sci U S A 2022 : 10.1073/pnas.2202842119