View primary antibody

Basic data

PID primary-0267
EPIC PID
Research group RG Betz
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol ACTN2
Antibody Registry ID(s) AB_307264
Name Anti-Sarcomeric Alpha Actinin antibody
Alternative name Actin binding protein antibody
Lab ID
Tag / Fluorophore
Raised in
Reacts with rat, human
Clone EA-53
Isotype IgG1
Clonality monoclonal
Demasking unknown
Antigen Full length native protein corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin
Crafted By company
Company / Manufacturer Abcam
Catalog no. ab9465
Lot no.
Description IHC-P Use a concentration of 10 - 20 µg/ml. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. Read More WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Localization Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
Storage instruction Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Receipt date 2021-04-01 00:00:00
Preparation date 2021-04-01 00:00:00
Created by
Last modified

External links

Applications


No application comments yet.

Linked publications


Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
Hofemeier AD et al.
eLife 2021: 10.7554/elife.60145