Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0267 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0267 |
| Research group | RG Betz |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | ACTN2 |
| Antibody Registry ID(s) | AB_307264 |
| Name | Anti-Sarcomeric Alpha Actinin antibody |
| Alternative name | Actin binding protein antibody |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | rat, human |
| Clone | EA-53 |
| Isotype | IgG1 |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Full length native protein corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab9465 |
| Lot no. | |
| Description | IHC-P Use a concentration of 10 - 20 µg/ml. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. Read More WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
| Localization | Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Receipt date | 2021-04-01 00:00:00 |
| Preparation date | 2021-04-01 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=ACTN2 |
| Wikipedia | http://en.wikipedia.org/wiki/ACTN2 |
| Uniprot | http://www.uniprot.org/uniprot/?query=ACTN2 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=ACTN2 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_307264 |
Applications
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Linked publications
Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
Hofemeier AD, Limón T, Muenker TM, Wallmeyer B, Jurado A, Afshar ME, Ebrahimi M, Tsukanov R, Oleksiievets N, Enderlein J, Gilbert PM, Betz T
eLife 2021 : 10.7554/elife.60145
