[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"954","pid":"primary-0954","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0954","research_group":"RG M\u00f6bius","lab_id":"","type":"primary","antigen_symbol":"ACTA1 ","antibody_registry_id":"AB_2223041","name":"Anti-Actin Antibody","alternative_name":"NEM3","tag_flurophore":"","raised_id":"Mouse","reacts_with":"all","clone":"C4","isotype":"IgG1\u03ba ","clonality":"monoclonal","demasking":"unknown","antigen":"Purified chicken gizzard actin","company":"Merck Millipore","catalog_no":"MAB1501","lot_no":"","description":"Indirect immunofluorescence at 1:100:\r\nTissue culture cells -- fix with formaldehyde, treat with methanol or acetone.\r\nGlycerinated myofibrils -- fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts.\r\nCryostat sections (6 \u00b5m) -- quick frozen in isopentane, slides treated with gelatin and formaldehyde.\r\n\r\nImmunoblots:\r\n1:100-1:1,000 (Otey, 1987):On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle.\r\n\r\nIodination (Lessard, 1979).\r\n\r\nSolid phase binding assay ELISA:\r\n1:800-1:1,000 dilution from a previous lot was shown to be strongly reactive with cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.\r\n\r\nELISA:\r\nstrongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.\r\n\r\nOptimal working dilutions must be determined by end user.","last_modified":", "}]