[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"848","pid":"primary-0848","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0848","research_group":"RG Fischer","lab_id":"","type":"primary","antigen_symbol":"RBFOX3","antibody_registry_id":"AB_2532109","name":"Recombinant Anti-NeuN antibody [EPR12763] - Neuronal Marker","alternative_name":"FOX-3","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"mouse, rat, sheep, goat, cat, dog, human, zebrafish, commonMarmoset","clone":"EPR12763","isotype":"IgG","clonality":"monoclonal","demasking":"unknown","antigen":"Synthetic peptide.","company":"Abcam","catalog_no":"ab177487","lot_no":"","description":"Flow Cyt (Intra) \t\t\r\n1\/100.\r\n\r\nab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.\r\n\r\nIHC (PFA fixed) \t\t\r\nUse at an assay dependent concentration.\r\n\r\nmIHC \t\t\r\n1\/1000.\r\n\r\nPerform Sodium citrate antigen retrieval (pH 6.0) in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.\r\n\r\nIHC-P \t\r\n1\/3000. Perform heat mediated antigen retrieval with Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol.\r\n\r\nSee IHC antigen retrieval protocols.\r\n\r\nFor unpurified use at 1\/800.\r\n\r\nWB \t\r\n1\/1000 - 1\/10000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 34 kDa).\r\n\r\nFor unpurified use at 1\/1000 - 1\/2000.\r\n\r\nICC\/IF \t\r\n1\/100 - 1\/300.\r\n\r\nFor unpurified use at 1\/80.\r\n\r\nIHC-Fr \t\r\nUse at an assay dependent concentration. ","last_modified":"Klauenberg, Vanessa"}]