[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"682","pid":"primary-0682","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0682","research_group":"RG Gollisch","lab_id":"","type":"primary","antigen_symbol":"AIF1 ","antibody_registry_id":"AB_2224402","name":"Anti-Iba1 antibody","alternative_name":"interferon gamma responsive transcript ","tag_flurophore":"","raised_id":"Goat","reacts_with":"rat, human","clone":"","isotype":"IgG","clonality":"polyclonal","demasking":"unknown","antigen":"Synthetic peptide corresponding to Human Iba1 aa 135-147 (C terminal)","company":"Abcam","catalog_no":"ab5076","lot_no":"","description":"IHC-P \t\r\nUse a concentration of 5 \u00b5g\/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.\r\nWB \t\r\nUse a concentration of 1 - 3 \u00b5g\/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa).\r\n\r\nA 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background.","last_modified":"Klauenberg, Vanessa"}]