[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"556","pid":"primary-0556","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0556","research_group":"RG Fischer","lab_id":"","type":"primary","antigen_symbol":"EOMES ","antibody_registry_id":"AB_778267","name":"Anti-TBR2 \/ Eomes antibody","alternative_name":"T-box brain2","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"mouse, human","clone":"","isotype":"IgG","clonality":"polyclonal","demasking":"unknown","antigen":"Corresponding to Mouse Eomes aa 650 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.","company":"Abcam","catalog_no":"ab23345","lot_no":"","description":"IHC-Fr \t\r\n1\/100 - 1\/500.\r\n\r\nAbcam recommends the following antigen retrieval method: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).\r\n\r\nWe recommend using Goat Anti-Rabbit IgG H\u0026amp;L (Alexa Fluor\u00ae 488) (ab150077) secondary antibody.\r\n\r\nWB \t\r\nUse a concentration of 0.4 - 2.5 \u00b5g\/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 72 kDa).\r\n\r\nAbcam recommends using milk as the blocking agent.\r\n\r\nIn our hands, when tested in western blot, this product typically gives a weaker signal in mouse tissue lysates compared to human cell lines. However, this product gives clean, specific staining in IHC-Fr on mouse E14.5 cortex. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented.","last_modified":"Klauenberg, Vanessa"}]