[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"378","pid":"primary-0378","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0378","research_group":"RG Fischer","lab_id":"","type":"primary","antigen_symbol":"H3C","antibody_registry_id":"AB_2118291","name":"Anti-Histone H3 (acetyl K27) antibody - ChIP Grade","alternative_name":"H3 histone family member E pseudogene antibody","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"mouse, cow, human, RecombinantFragment","clone":"","isotype":"IgG","clonality":"polyclonal","demasking":"unknown","antigen":"Synthetic peptide corresponding to Human Histone H3 aa 1-100 (acetyl K27) conjugated to keyhole limpet haemocyanin.","company":"Abcam","catalog_no":"ab4729","lot_no":"","description":"ICC\/IF\tUse a concentration of 0.5 \u00b5g\/ml. Can be used with paraformaldehyde- or methanol- fixed cells.\r\nWB\t  Use a concentration of 1 \u00b5g\/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). We recommend Goat Anti-Rabbit IgG H\u0026amp;L (HRP) (ab97051) secondary antibody.\r\nIHC-P    Use a concentration of 1 \u00b5g\/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.\r\nChIP    Use 2 \u00b5g for 25 \u00b5g of chromatin. We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control.\r\nPepArr\t\tUse a concentration of 0.2 - 0.02 \u00b5g\/ml.","last_modified":"Klauenberg, Vanessa"}]