[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"377","pid":"primary-0377","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0377","research_group":"RG Fischer","lab_id":"","type":"primary","antigen_symbol":"H3C1","antibody_registry_id":"AB_306847","name":"Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade","alternative_name":"H3 histone family member E pseudogene antibody","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"cow, human","clone":"","isotype":"IgG","clonality":"polyclonal","demasking":"unknown","antigen":"Synthetic peptide within Human Histone H3 aa 1-100 (mono methyl K4) conjugated to keyhole limpet haemocyanin. ","company":"Abcam","catalog_no":"ab8895","lot_no":"","description":"ICC\/IF\tUse a concentration of 1 \u00b5g\/ml. Works better if cells are fixed with methanol.\r\nChIP\tUse 2 \u00b5g for 25 \u00b5g of chromatin. We recommend Myo-D ChIP primer pair ab269261 as positive control.\r\nWB\t    1\/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).\r\nIHC-P    Use a concentration of 0.5 \u00b5g\/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.","last_modified":"Klauenberg, Vanessa"}]