[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"367","pid":"primary-0367","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0367","research_group":"RG Fernandez-Busnadiego","lab_id":"","type":"primary","antigen_symbol":"SNCA","antibody_registry_id":"AB_869973","name":"Recombinant Anti-Alpha-synuclein (phospho S129) antibody","alternative_name":"Alpha synuclein antibody","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"mouse, rat, human","clone":"EP1536Y","isotype":"IgG","clonality":"monoclonal","demasking":"unknown","antigen":"Synthetic peptide within Human Alpha-synuclein aa 100 to the C-terminus. The exact sequence is proprietary.","company":"Abcam","catalog_no":"ab51253","lot_no":"","description":"IHC-FrFl\tUse at an assay dependent concentration.\r\nWB\t1\/1000 - 1\/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826). Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.\r\nDot blot\t\tUse at an assay dependent concentration.\r\nELISA\t\tUse at an assay dependent concentration.\r\nIHC-P\t\tUse a concentration of 5 - 10 \u00b5g\/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.","last_modified":"Klauenberg, Vanessa"}]