[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"366","pid":"primary-0366","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0366","research_group":"RG Fernandez-Busnadiego","lab_id":"","type":"primary","antigen_symbol":"SQSTM1","antibody_registry_id":"AB_945626","name":"Anti-SQSTM1 \/ p62 antibody","alternative_name":"A170 antibody","tag_flurophore":"","raised_id":"Mouse","reacts_with":"human","clone":"2C11","isotype":"IgG2a","clonality":"monoclonal","demasking":"unknown","antigen":"Recombinant full length protein corresponding to Human SQSTM1\/ p62 aa 1-440.","company":"Abcam","catalog_no":"ab56416","lot_no":"","description":"IHC-P   Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.\r\nWB\tUse at an assay dependent concentration.\r\nICC\/IF   Use at an assay dependent concentration. Fix cells for 15 minutes with 2 mL of 4% paraformaldehyde solution (pH 7.4 with NaOH in PBS). Permeabilize cells by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS.\r\nFlow Cyt\t\t\r\nUse at an assay dependent concentration.\r\nab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.\r\n\r\nWe recommend Goat Anti-Mouse IgG H\u0026amp;L (DyLight\u00ae 488) preadsorbed (ab96879) secondary antibody","last_modified":"Klauenberg, Vanessa"}]