[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"96","pid":"primary-0096","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0096","research_group":"RG Zimmermann","lab_id":"","type":"primary","antigen_symbol":"AQP1","antibody_registry_id":"AB_1163380","name":"Anti-Aquaporin 1 Antibody","alternative_name":"CHIP28","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"human, rat, mouse","clone":"","isotype":"","clonality":"polyclonal","demasking":"unknown","antigen":"KLH-conjugated linear peptide corresponding to the topological domain of rat Aquaporin 1.","company":"Merck Millipore","catalog_no":"AB2219","lot_no":"","description":"Western Blotting: 1-10 \u03bcg\/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith \u0026amp; Agre 1991) We recommend the use of 0.5-1.0% milk in all primary\/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.\r\nImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aquaporin 1 in human kidney and mouse kidney tissue.","last_modified":"Klauenberg, Vanessa"}]